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International Journal of Analytical Chemistry
Volume 2017 (2017), Article ID 1670812, 6 pages
Research Article

Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal

1Department of Environmental Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China
2Department of Chemistry, College of Pharmacy, Third Military Medical University, Chongqing 400038, China

Correspondence should be addressed to Huawen Zhao and Weiqun Shu

Received 19 February 2017; Revised 13 April 2017; Accepted 7 May 2017; Published 25 May 2017

Academic Editor: Guannan Wang

Copyright © 2017 Liping Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A rapid, simple, and novel method for folate receptor α (FRα) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FRα antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FRα was added to the probe for the specific interaction between antibody and antigen, resulting in the enhancement of resonance Rayleigh scattering (RRS) intensity. There is a linear relationship between the change of RRS intensity () and the concentration of FRα, with the detecting range of 0.50–37.50 ng·mL−1 and the limit of determination of 0.05 ng·mL−1. The determination of FRα in serum samples was realized with the advantages of high selectivity, high sensitivity, and easy operation.