Research Article

NMR Confirmation and HPLC Quantification of Javamide-I and Javamide-II in Green Coffee Extract Products Available in the Market

Table 1

(a) The amounts of javamide-I in 1st and 2nd extractions. The mixture of seven green coffee extract samples ((1) Nature Made Inc., (2) BioRise Nutrition, (3) GMX Naturals, (4) Natrogix, (5) Pro Nutrient Labs, (6) The Health Life, and (7) Wellsome Nutrition) was extracted twice with 70% MeOH (50 mL), providing the 1st and 2nd extracts. The amounts of javamide-I were presented as mg per 1 g green coffee sample (). (b) The recovery yield of javamide-I. The purified javamide-I (0.2 mg) was added to the mixture of the seven green coffee extract samples containing 0.2 mg javamide-I and extracted with 70% MeOH. The recovery was determined using 1st and 2nd extracts ().
(a)

Sample1st (mg)2nd (mg) 1st and 2nd (mg)1st/2nd (%)

S1–S795.0

(b)

SampleInitial (mg)J- (mg)1st (mg)2nd (mg)Yield (%)

S1–S70.20.298.0