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International Journal of Analytical Chemistry
Volume 2017, Article ID 2397131, 5 pages
Research Article

Quantification of Barbatusin and 3β-Hydroxy-3-deoxybarbatusin in Plectranthus Species by HPLC-DAD

Programa de Pós-Graduação em Química, Universidade Federal do Ceará, Campus do Pici, 60455-970 Fortaleza, CE, Brazil

Correspondence should be addressed to Jair Mafezoli; rb.cfu@zefamj

Received 23 April 2017; Accepted 4 June 2017; Published 3 July 2017

Academic Editor: Neil D. Danielson

Copyright © 2017 Maria Goretti de Vasconcelos Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The concentration of diterpenes barbatusin (1) and 3β-hydroxy-3-deoxybarbatusin (2) in the extracts from leaves of Plectranthus grandis, P. barbatus, P. ornatus, and P. amboinicus was evaluated by HPLC-DAD analysis on a Luna C-18 column, using isocratic mixtures of water and acetonitrile as eluents. The regression equations were obtained with good linearity (r2 > 0.99) and limit of quantifications was higher than 0.1 µg/mL. The precision (lower than 3.5%, within day) and accuracy (higher than 81.7% and lower than 107.6%) of the methods were adequate. Barbatusin (1) was detected in P. grandis (15.432 ± 2.28 mg/g) and P. barbatus (5.198 ± 3.45 mg/g) extracts, while compound 2 was detected in P. grandis (4.068 ± 3.34 mg/g), P. barbatus (0.654 ± 5.86 mg/g), P. amboinicus (0.160 ± 7.25 mg/g), and P. ornatus (0.763 ± 5.10 mg/g). The evaluated validation parameters were satisfactorily achieved, and the developed methodology represents a suitable tool for application in the quantification of barbatusin (1) and 3β-hydroxy-3-deoxybarbatusin (2) in Plectranthus species.