Phenolics in Primula veris L. and P. elatior (L.) Hill Raw Materials

Table 1

Validation parameters of the HPLC-DAD analysis ().

Compound	Purity (%)	Precision intraday (CV, %)	Calibration equation	Linearity ()	Linear range (mg/mL)	LOD (µg/L)	LOQ (µg/L)
/+/-Catechin	98,3	0.80		0.9995	0.492–49.150	11.07	36.10
Luteolin 8-C-glucoside (orientin)	99,4	1.12		0.9998	0.497–49.700	36.84	122.83
Quercetin 3-O-rutinoside (rutoside)	91,4	1.61		0.9999	0.907–90.669	74.60	248.80
Quercetin 3-O-galactoside (hyperoside)	95,0	1.48		0.9999	0.384–38.400	35.16	117.20
Isorhamnetin-3-O-rutinoside	95,1	1.09		0.9998	0.380–38.000	29.31	97.69
Isorhamnetin-3-O-glucoside	95,3	1.02		0.9998	0.380–38.000	33.90	113.00
Kaempferol 3-O-glucoside (astragalin)	99,4	1.68		0.9999	0.410–81.906	33.00	109.90
3-O-Caffeoylquinic acid (chlorogenic acid)	98,64	1.24		0.9997	0.395–39.456	20.97	69.90
Primverin	95,0	1.18		0.9999	0.407–40.660	7.95	26.52
Primulaverin	95,2	0.96		0.9999	0.395–39.520	38.39	127.96