Research Article

Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone

Table 1

Comparison of presented assay with Ellman’s assay and literature.

Detection limitFabrication timeAssay timeNecessary equipment

Presented smartphone assayGalanthamine: 149.1 nM
Donepezil: 22.3 nM
Aprox. 1 hour15 minSmartphone
Standard Ellman’s assayGalanthamine: 18.3 nM
Donepezil: 3.82 nM
NA10 minSpectrophotometer
Spectrophotometric assay [21]Galanthamine: 0.05 nMAprox. 3.5 hoursAprox. 20 mnSpectrophotometer
Potentiometric assay [22]Galanthamine: 5.4 nMAprox. 10 hoursAprox. 10 minElectrodes, potentiometer
Square-wave voltammetry [23]Donepezil: 151 nMNAAprox. 20 minElectrodes, electrochemical analyser