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International Journal of Analytical Chemistry
Volume 2017, Article ID 3813879, 6 pages
https://doi.org/10.1155/2017/3813879
Research Article

Analysis of Three Compounds in Flos Farfarae by Capillary Electrophoresis with Large-Volume Sample Stacking

1Translational Medicine Research Center, Shanxi Medical University, Taiyuan 030001, China
2Pharmacy Department, Shanxi Boai Hospital, Taiyuan 030001, China
3School of Pharmacy, Shanxi Medical University, Taiyuan 030001, China

Correspondence should be addressed to Hong-Fen Zhang; moc.621@7002_fhgnahz and An-jia Chen; moc.361@888aijnanehc

Received 6 December 2016; Revised 10 April 2017; Accepted 12 June 2017; Published 5 September 2017

Academic Editor: Günther K. Bonn

Copyright © 2017 Hai-xia Yu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to develop a method combining an online concentration and high-efficiency capillary electrophoresis separation to analyze and detect three compounds (rutin, hyperoside, and chlorogenic acid) in Flos Farfarae. In order to get good resolution and enrichment, several parameters such as the choice of running buffer, pH and concentration of the running buffer, organic modifier, temperature, and separation voltage were all investigated. The optimized conditions were obtained as follows: the buffer of 40 mM NaH2P04-40 mM Borax-30% v/v methanol (pH 9.0); the sample hydrodynamic injection of up to 4 s at 0.5 psi; 20 kV applied voltage. The diode-array detector was used, and the detection wavelength was 364 nm. Based on peak area, higher levels of selective and sensitive improvements in analysis were observed and about 14-, 26-, and 5-fold enrichment of rutin, hyperoside, and chlorogenic acid were achieved, respectively. This method was successfully applied to determine the three compounds in Flos Farfarae. The linear curve of peak response versus concentration was from 20 to 400 µg/ml, 16.5 to 330 µg/mL, and 25 to 500 µg/mL, respectively. The regression coefficients were 0.9998, 0.9999, and 0.9991, respectively.