Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions

Table 1

Validation parameters describing the diphenhydramine analysis method.

Linearity range [μg/spot]		LOD [μg/spot]		0.12
Intercept	235.35 (±93.54)	LOQ [μg/spot]		0.35
Slope	4099.28 (±94.39)		Accuracy (% of recovery)	Precision, RSD [%]
Standard deviation	143.59	80%	106.7	1.77
Determination coefficient	99.79%	100%	108.0	1.58
Significance of regression	2937	120%	107.3	1.45