Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection

Table 3

Accuracy of HPLC system used for glucosinolates determination.

Analyte	Prepared concentration; (µmoles mL^−1)	Measured concentration; (µmoles mL^−1)	Recovery (%)	Recovery (mean ± % RSD)
Sinigrin	300	325.9	108.6	108.6 ± 0.17
	300	326.5	108.8
	300	325.4	108.5
	150	152.5	101.7	101.3 ± 0.32
	150	151.7	101.1
	150	151.8	101.2
	75	66.9	89.3	89.0 ± 0.19
	75	66.7	88.9
	75	66.7	88.9
Glucotropaeolin	300	302.4	100.8	100.5 ± 0.41
	300	300.1	100.0
	300	302.0	100.7
	150	153.5	102.3	102.4 ± 0.03
	150	153.6	102.4
	150	153.6	102.4
	75	71.9	95.9	95.7 ± 0.31
	75	71.9	95.8
	75	71.5	95.3