Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection

Table 4

Glucosinolates identified in the current Chinese hairy roots. Respective retention time (Rt). Wavelengths of maximum absorption in the visible region by elution order and respective average content.¹

GLS identified	Chemical formula & structure	Rt; (min)	UV; (nm)	HPLC-DAD-UV/Vis band; (nm)	Average level; (µmoles⋅100 g^−1 dw)
Glucobrassicin		17.07	229	221	409.75
4-Methoxyglucobrassicin		19.21	229	222	182.15
1-Methoxyglucobrassicin (neoglucobrassicin)		23.38	229	224	195.06

value of glucosinolate content is expressed as mean of three replicates.