Development of a Rapid and Simple Method to Remove Polyphenols from Plant Extracts

Figure 2

The absorbance (760 nm) obtained for Folin-Ciocalteu assay for polyphenols in different fractions of black tea extract obtained after treatment with PVPP columns containing 0.9, 0.7, and 0.3 g (a), absorbance (760 nm) of gallic acid (100 μg/mL), vanillin (100 μg/mL), epigallocatechin gallate (EGCG) (100 μg/mL), and fresh tea flush (200 μg/mL) after treatment with PVPP column (0.9 g) for Folin-Ciocalteu assay (b), and absorbance at 280 nm for GA (100 μg/mL), vanillin (100 μg/mL), and epigallocatechin gallate (EGCG) (100 μg/mL) (c). Fraction number “0” is the absorbance obtained for fresh tea flush extract before the PVPP treatment. The values are expressed as Mean ± SD. Number of replicates for each experiment are given in the text.

(a)

(b)

(c)