Green Analytical Methods for the Separation of Seven Antihistamines: Application in Separation of Azelastine and Related Impurities in Nasal Solution

Table 7

System suitability parameters obtained in the separation of azelastine and related impurities.

	Retention factor; (k’)	Peak resolution (Rs)	Peak asymmetry; (As)
Impurity B	-0.35		1.36
Azelastine	0.43	9.0 (B-Aze)	1.36
Impurity D	3.04	17.14 (Aze-D)	1.14
Impurity E	5.04	13.14 (D-E)	1.05

k’: relative retention (retention factor).
Rs: resolution between adjacent peaks.