Research Article
Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines
Figure 4
Expression of TPM1B transcripts in breast epithelial cell lines. cDNA from each cell line was amplified with the TPM1. Exon 1a(+)/TPM1. Exon 9d(−) primer pair (Table 1). Amplified DNAs were run on an agarose gel (Panel (a)) and subsequent hybridization was carried out using TPM1 exon 2a-specific 32P-labeled oligonucleotide probe (Panel (b)). Panel (c) depicts the ethidium bromide staining of the DNA amplified from human GAPDH cDNAs from each of the cell lines. Lane 1, MCF7; lane 2, MCF10A; lane 3, HCC1143; lane 4, HCC1143BL; lane 5, MDAMB453; lane 6, MDAMB468; lane 7, BT474; lane 8, HCC1187; lane 9, HCC1806; lane 10, MDAMB157; lane 11, HCC1419; lane 12, MDAMB1187BL; lane M, DNA marker; lane 13, primer only control.
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