snRNP: Rich Nuclear Bodies in Hyacinthus orientalis L. Microspores and Developing Pollen Cells
Figure 1
Hyacinthus orientalis L. silver nitrate-stained microspore and developing pollen grains (N-nucleus, No - nucleolus, VN-vegetative nucleus, VC-vegetative cytoplasm, GN-generative nucleus, GC-generative cytoplasm). Bars-10 m. Figure 1. In the nucleus of the microspore there are clearly visible three extranucleolar agyrophylic bodies (arrowheads). Figure 1a. Just after the microspore mitosis, in the vegetative nucleus a nucleolus and three regular bodies can be seen (arrowheads). In the generative nucleus, excluding nucleolus, one regular and strongly agyrophylic body is present (arrowhead). Figure 1b. In both nuclei of young pollen grain nucleolus can be observed. In the vegetative nucleoplasm two silver nitrate-stained bodies are visible (arrowheads). Figure 1c. Pollen grain after detachment of the generative cell from the sporoderm is shown. The vegetative nucleus is with an evident nucleolus and one nuclear body (arrowhead). The generative nucleus exhibits strong Ag-NOR staining, without organized nucleolus and nuclear bodies. Figure 1c’. Magnification of generative nucleus is shown on Figure 1c (area marked with the dashed line). Disperse, highly agyrophylic nucleolar material can be seen in the center of the generative cell. Figure 1d. Premature pollen grain is shown. Only highly agyrophylic nucleolus can be observed in the vegetative nucleoplasm. The generative nucleus shows strong staining. Figure 1d’. Magnification of generative nucleus is shown on Figure 1d (area marked with the dashed line). Strongly Ag-NOR stained nucleolar material is localized in the whole area of the nucleoplasm. Figure 1e. Pollen grain just before antheisis is shown. Highly agyrophylic, flapped-shape vegetative nucleus, without organized nucleolus can be seen. Spindle-shape generative nucleus exhibits the presence of intense silver nitrate staining in the form of irregular clusters. Figure 1e’. Magnification of vegetative nucleus is shown on Figure 1e (area marked with the dashed line). Nucleolar material, in the form of dark clusters, can be seen in the central area of the nucleus
