snRNP: Rich Nuclear Bodies in Hyacinthus orientalis L. Microspores and Developing Pollen Cells

Figure 3

Concentration of snRNP-indicating molecules in nuclear bodies of microspore and young pollen cells showed using immunofluorescence techniques (N-nucleus, No - nucleolus, VN-vegetative nucleus, VC-vegetative cytoplasm, GN-generative nucleus, GC-generative cytoplasm). Figures 3’–3g’. DAPI staining of given microspores and pollen grains are shown. Bars-10 𝜇m. Figure 3. Polarized microspore is shown. Immunofluorescence localization of TMG snRNA indicating its high accumulation in the nuclear body (arrowhead) adjacent to the nucleolus is shown. Figure 3a. Nuclear body containing high levels of Sm proteins (arrowhead) can be seen in the microspore nucleoplasm. Figures 3b and 3b’ show the magnification of the area marked with the dashed line on Figures 3a and 3a’. No DNA labelling can be observed in the area of the nuclear body (arrowheads). Figure 3c. High concentration of U2 snRNA is visible in two nuclear bodies (arrowheads) adjacent to the nucleolus in microspore nucleus. Figure 3d. Pollen grain just after microspore mitosis is shown. Nuclear body containing high levels of TMG snRNA is indicated in the vegetative nucleoplasm (arrowhead). Figures 3e and 3e’ show the magnification of the area marked with the dashed line on Figures 3d and 3d’. The lack of DNA can be observed in the nuclear body area (arrowheads). Figure 3f. Young pollen grain is shown. Two Sm-rich nuclear bodies are present in the vegetative nucleus (arrowheads). Figure 3g. In the vegetative and generative nucleus of young pollen grain one nuclear body, containing U2 snRNA is indicated (arrowheads). Figure 3h. Control reaction of fluorescence in situ hybridization performed with the U2 snRNA sense probe (N-nucleus, C-cytoplasm) is shown. No labeling can be seen in the nucleus of polarized microspore. Figure 3h’. DAPI staining of microspore is shown on Figure 3h.