snRNP: Rich Nuclear Bodies in Hyacinthus orientalis L. Microspores and Developing Pollen Cells

Figure 4

Immunogold localization of snRNP-indicating molecules in nuclear bodies of microspore and vegetative cell of young pollen grain (N-nucleus, No - nucleolus, VN-vegetative nucleus). Bars-0.5 𝜇m. Figure 4. Immunogold labelling of TMG snRNA in the vegetative nucleus of young pollen grain is shown. Strong labelling can be observed in the nuclear body (arrowhead) localized in close proximity to the nucleolus. Figure 4a. Ultrastructural Sm protein localization in the vegetative nucleus of young pollen grain is shown. Numerous gold particles are concentrated over the nuclear body (arrowhead). Figure 4b. In situ hybridization to U2 snRNA is shown. The presence of U2 snRNA over the nuclear body can be seen in the microspore nucleus (arrowhead). Figure 4c. DNA labelling using TdT technique in polarized microspore nucleus (N-nucleus) is shown. No labelling can be seen in the nuclear body (arrowhead). Figure 4d. Control reaction of in situ hybridization performed using U2 snRNA sense probe (N-nucleus, No-nucleolus) is shown. No gold particles can be seen over the nucleoplasm and nuclear body (arrowhead) localized in close proximity to the nucleolus.