Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes
Figure 1
BMP-4 decreases the protein levels of TRP-1, and MC1-R. Paired cultures of melanocytes were treated with either vehicle or BMP-4 (25 ng/mL). Cells were then harvested at 24 and 48 hours for immunoblot analysis using monoclonal antibodies specific for PKC- (a), TRP-1 (b), and MC1-R (c). The protein level of actin was used for the loading control. The level of PKC- protein was decreased by 49.9 ±29.7% () at 24 hours and at 48 hours by 47.7 ±18% () [23, 27]. The levels of TRP-1 was decreased by 45% ±5.0% () and 36 ±6.5.0% () at 24 and 48 hours time points, respectively (Figure 1(b)). The protein level of MC1-R was decreased by 50% ±2.0% () and 55% ±2.5% () at 24 and 48 hours, respectively (Figure 1(c)). A representative result from five separate experiments is shown.
