Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of -Adrenergic Receptors to Phosphoinositide 3-Kinase
Figure 7
Pertussis toxin effects on β-AR-mediated PI-3K activation. Twenty-six
hours after transfection, cells were incubated with 0.1 μg/mL pertussis toxin
for 18 hours. Cells were stimulated for 5 minutes with 1 μM isoproterenol prior
to processing for TLC as described in Figure 1 and Section
2. PI-3K
activity was determined by the level of -PI produced by the stimulation of -AR (a), -AR (b),
-AR (c), and
-AR expressing cells. (; versus basal and PTX-Iso conditions).
