Regulatory T Cells Resist Cyclosporine-Induced Cell Death via CD44-Mediated Signaling Pathways
CD44 cross-linking promotes Foxp3 expression in an IL-2-independent manner. (a) Fold Increase (FI) in GFP/FoxP3 MFI for Treg activated with anti-CD3 and anti-CD28 Ab alone or in conjunction with plate-bound anti-CD44 Ab with or without anti-IL-2 Ab (Anti-IL-2), recombinant CD25 (rCD25), or IL-2 (). (b) Representative histograms demonstrating GFP/Foxp3 expression by Treg isolated from GFP/Foxp3 knock-in mice on a conventional B6 background mice or on a CD25−/− background (B6 GFP/Foxp3.CD25−/− mice) following 3 days of culture with anti-CD3 and anti-28 alone or in conjunction with plate-bound anti-CD44. (c) Representative histograms illustrating GFP/FoxP3 expression of Treg following 3 days of culture with anti-CD3 and anti-CD28 alone, or in conjunction with plate-bound CD44 Ab, and with or without varying doses of IL-2. Data are representative of two experiments.