Figure 1: TPO primers’ selection. (a, b) Schematic illustration of human TPO gene and mRNA isoforms and selected TPO primers. (a) TPO gene contains 6 exons (E 1–6) and 5 introns (I 1–5). (b) Alternative RNA splicing patterns previously identified for TPO. Horizontal arrows represent the amplified regions by RT-PCR. (c) Primers used for PCR and nested-PCR. First PCR products were used as DNA template for the nested-PCR. TPO-amplified isoforms and their sizes are shown. GADPH PCR was used as control. (d) TaqMan Probes for TPO and GADPH.