Figure 4: Comparison of TPO-3 sequence as well as TPO levels in the soluble cancer cells extracts. (a) Sequences of PCR products extracted from agarose gel bands are shown in the upper line. R2 primer was used for sequencing. Boxes show a C/T 5183 SNP. “…” and “” symbols refer to two different alternative splicing sites (12 bp and 116 bp deletions, resp.). A 116 bp deletion characterizing TPO-3 is detected. BLAST reveals no significant mutation. (b) TPO levels in the soluble cancer cells extracts evaluated by enzyme-linked immunosorbent assay (ELISA) for 106 cell/ml (the mean of three experiments) from OVCAR-3 NIH cell line, breast cancer MDA-MB231 cell line (as control), myeloid leukemia K562, promyelocytic leukemia HL60 cell lines, and primary ovarian carcinomatosis ().