Class-Specific Histone Deacetylase Inhibitors Promote 11-Beta Hydroxysteroid Dehydrogenase Type 2 Expression in JEG-3 Cells

Figure 3

Class-specific HDACs on 11β-HSD2 regulation in the placenta. (a) Expression data from the BioGPS database showing the relative expression of class I HDAC, HDAC 1 in the placenta (red) relative to multiple human tissues and fetal brain. Graphical representation of (b) 11β-HSD2 and (c) AcH3 expression in JEG-3 cells treated with 0–10 μM of class I HDAC inhibitor MS275 for 24 h. (d) Representative photomicrographs of JEG-3 cells immunocytochemically stained for 11β-HSD2 and AcH3 after treatment with (0–10 μM) MS275 for 24 h. (e) Expression data from the BioGPS database showing the relative expression of class II HDAC, HDAC 5 in the placenta (red) relative to multiple human tissues and fetal brain. Graphical representation of (f) 11β-HSD2 and (g) AcH3 expression in JEG-3 cells treated with 0–10 mM of class IIa HDAC inhibitor MC1568 for 24 h. Data are expressed as mean ± SEM. (h) Representative photomicrographs of JEG-3 cells immunocytochemically stained for 11β-HSD2 and AcH3 after treatment with (0–10 μM) MC1568 for 24 h. Data are expressed as mean ± SEM ( compared to 0 μM; (b, f) one-way ANOVA with post hoc Tukey’s and (c, g) unpaired Student’s -test; 25 cells per group per experiment; ). Scale bar = 50 μm.

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