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Figure 4: Cortisol and IL-1β response in JEG-3 cells. (a) Representative photomicrographs of JEG-3 cells immunocytochemically stained for the glucocorticoid receptor (GR; green) and (c) the interleukin 1 receptor, type I (IL-1R1; red). The second panel shows the corresponding DAPI stained image. (b, c) MTT assay examining the viability of JEG3 cells treated with either 0–10 μM cortisol (b) or 0–100 ng/ml IL-1β (c) for 24 h in vitro. (d) Graphical representation showing the levels 11β-HSD2 in JEG-3 cells exposed to a vehicle (control), 10 ng/ml IL-1β or 2 μM cortisol for 24 h. Data are expressed as mean ± SEM ( compared to 0 μM, compared to control; one-way ANOVA with post hoc Tukey’s; (d) 100 cells per group per experiment; ). Scale bar = 50 μm.