Table of Contents
International Journal of Carbohydrate Chemistry
Volume 2011 (2011), Article ID 146419, 11 pages
http://dx.doi.org/10.1155/2011/146419
Research Article

Selective Modification of Chitosan to Enable the Formation of Chitosan-DNA Condensates by Electron Donator Stabilization

Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, 207 Othmer Hall, Lincoln, NE 68588-0643, USA

Received 2 February 2011; Revised 4 May 2011; Accepted 31 May 2011

Academic Editor: José das Neves

Copyright © 2011 Karl E. Kador and Anuradha Subramanian. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chitosan, a polyaminosaccharide, has been investigated for its use in the field of drug-delivery and biomaterial applications because of its natural biocompatibility and polycationic properties. Chemical modifications of chitosan have been attempted in an effort to increase the transfection efficiency with respect to gene delivery applications; however, it is unknown how these modifications affect the formation of the condensates. This study attempts to determine the effects of modification of the cationic center of chitosan on the ability to condense DNA. Specifically, electron-donating or -withdrawing groups were used as modifiers of the cationic charge on the chitosan backbone to stabilize the protonated form of chitosan, which is necessary to form condensates and increase the efficiency of the polymer to condense DNA by yielding condensates at a lower nitrogen to phosphorous (N : P) ratio. While an N : P ratio of 7 is needed to condense DNA with unmodified chitosan, phthalate-modified chitosan yielded condensates were obtained at an N : P ratio of 1.0.