Table of Contents
International Journal of Carbohydrate Chemistry
Volume 2011, Article ID 303708, 9 pages
http://dx.doi.org/10.1155/2011/303708
Review Article

Chitosan-Based Macromolecular Biomaterials for the Regeneration of Chondroskeletal and Nerve Tissue

1Institute for Composite and Biomedical Materials (IMCB), Research Unit of Pisa, Italian National Research Council (CNR), Largo Lucio Lazzarino, 56122 Pisa, Italy
2Department of Chemical Engineering, Industrial Chemistry and Materials Science (DICCISM), University of Pisa, Largo Lucio Lazzarino, 56122 Pisa, Italy

Received 1 February 2011; Revised 26 April 2011; Accepted 21 June 2011

Academic Editor: Alejandro Sosnik

Copyright © 2011 Giulio D. Guerra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. Berger, M. Reist, J. M. Mayer, O. Felt, N. A. Peppas, and R. Gurny, “Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 57, no. 1, pp. 19–34, 2004. View at Publisher · View at Google Scholar · View at Scopus
  2. C. Tangsadthakun, S. Kanokpanont, N. Sanchavanakit et al., “The influence of molecular weight of chitosan on the physical and biological properties of collagen/chitosan scaffolds,” Journal of Biomaterials Science, vol. 18, no. 2, pp. 147–163, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. R. A. A. Muzzarelli, “Immobilization of enzymes on chitin and chitosan,” Enzyme and Microbial Technology, vol. 2, no. 3, pp. 177–184, 1980. View at Google Scholar · View at Scopus
  4. P. Cerrai, G. D. Guerra, M. Tricoli, S. Maltinti, N. Barbani, and L. Petarca, “Polyelectrolyte complexes obtained by radical polymerization in the presence of chitosan,” Macromolecular Chemistry and Physics, vol. 197, no. 11, pp. 3567–3579, 1996. View at Google Scholar · View at Scopus
  5. G. D. Guerra, P. Cerrai, M. Tricoli, S. Maltinti, and R. S. Del Guerra, “In vitro cytotoxicity testing of chitosan-containing polyelectrolyte complexes,” Journal of Materials Science: Materials in Medicine, vol. 9, no. 2, pp. 73–76, 1998. View at Publisher · View at Google Scholar · View at Scopus
  6. R. A. A. Muzzarelli, G. Barontini, and R. Rocchetti, “Immobilized enzymes on chitosan columns: α chymotrypsin and acid phosphatase,” Biotechnology and Bioengineering, vol. 18, no. 10, pp. 1445–1454, 1976. View at Google Scholar · View at Scopus
  7. R. A. A. Muzzarelli, G. Barontini, and R. Rocchetti, “Isolation of lysozyme on chitosan,” Biotechnology and Bioengineering, vol. 20, no. 1, pp. 87–94, 1978. View at Google Scholar · View at Scopus
  8. R. A. A. Muzzarelli and O. Tubertini, “Chitin and chitosan as chromatographic supports and adsorbents for collection of metal ions from organic and aqueous solutions and sea-water,” Talanta, vol. 16, no. 12, pp. 1571–1577, 1969. View at Google Scholar · View at Scopus
  9. R. A. A. Muzzarelli and R. Rocchetti, “The use of chitosan columns for the removal of mercury from waters,” Journal of Chromatography A, vol. 96, no. 1, pp. 115–121, 1974. View at Google Scholar · View at Scopus
  10. R. A. Muzzarelli, A. Isolati, and A. Ferrero, “Chitosan membranes,” Ion Exchange and Membranes, vol. 1, no. 4, pp. 193–196, 1974. View at Google Scholar · View at Scopus
  11. R. A. A. Muzzarelli, C. Zucchini, P. Ilari et al., “Osteoconductive properties of methyl-pyrrolidinone chitosan in an animal model,” Biomaterials, vol. 14, no. 12, pp. 925–929, 1993. View at Publisher · View at Google Scholar · View at Scopus
  12. K. H. Bodek and A. Kufelnicki, “Interaction of microcrystalline chitosan with Ni(II) and Mn(II) ions in aqueous solution,” Journal of Applied Polymer Science, vol. 98, no. 6, pp. 2572–2577, 2005. View at Publisher · View at Google Scholar · View at Scopus
  13. R. A. A. Muzzarelli and C. Muzzarelli, “Chitosan chemistry: relevance to the biomedical sciences,” Advances in Polymer Science, vol. 186, pp. 151–209, 2005. View at Publisher · View at Google Scholar · View at Scopus
  14. I. Y. Kim, S. J. Seo, H. S. Moon et al., “Chitosan and its derivatives for tissue engineering applications,” Biotechnology Advances, vol. 26, no. 1, pp. 1–21, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. M. Amaike, Y. Senoo, and H. Yamamoto, “Sphere, honeycomb, regularly spaced droplet and fiber structures of polyion complexes of chitosan and gellan,” Macromolecular Rapid Communications, vol. 19, no. 6, pp. 287–289, 1998. View at Google Scholar · View at Scopus
  16. H. Yamamoto and Y. Senoo, “Polyion complex fiber and capsule formed by self-assembly of chitosan and gellan at solution interfaces,” Macromolecular Chemistry and Physics, vol. 201, no. 1, pp. 84–92, 2000. View at Google Scholar · View at Scopus
  17. K. Ohkawa, M. Yamada, A. Nishida, N. Nishi, and H. Yamamoto, “Biodegradation of chitosan-gellan and poly(L-lysine)-gellan polyion complex fibers by pure cultures of soil filamentous fungi,” Journal of Polymers and the Environment, vol. 8, no. 2, pp. 59–66, 2000. View at Publisher · View at Google Scholar · View at Scopus
  18. R. A. A. Muzzarelli, M. Mattioli-Belmonte, A. Pugnaloni, and G. Biagini, “Biochemistry, histology and clinical uses of chitins and chitosans in wound healing,” in Chitin and Chitinases, P. Jollès and R. A. A. Muzzarelli, Eds., pp. 251–264, Birkhäuser, Basel, Switzerland, 1999. View at Google Scholar
  19. B. A. Zielinski and P. Aebischer, “Chitosan as a matrix for mammalian cell encapsulation,” Biomaterials, vol. 15, no. 13, pp. 1049–1056, 1994. View at Publisher · View at Google Scholar · View at Scopus
  20. J. E. Babensee, L. V. McIntire, and A. G. Mikos, “Growth factor delivery for tissue engineering,” Pharmaceutical Research, vol. 17, no. 5, pp. 497–504, 2000. View at Publisher · View at Google Scholar · View at Scopus
  21. S. Sampaio, P. Taddei, P. Monti, J. Buchert, and G. Freddi, “Enzymatic grafting of chitosan onto Bombyx mori silk fibroin: kinetic and IR vibrational studies,” Journal of Biotechnology, vol. 116, no. 1, pp. 21–33, 2005. View at Publisher · View at Google Scholar · View at Scopus
  22. G. Freddi, A. Anghileri, S. Sampaio, J. Buchert, P. Monti, and P. Taddei, “Tyrosinase-catalyzed modification of Bombyx mori silk fibroin: grafting of chitosan under heterogeneous reaction conditions,” Journal of Biotechnology, vol. 125, no. 2, pp. 281–294, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. A. B. Kayitmazer, D. Shaw, and P. L. Dubin, “Role of polyelectrolyte persistence length in the binding of oppositely charged micelles, dendrimers, and protein to chitosan and poly(dimethyldiallyammonium chloride),” Macromolecules, vol. 38, no. 12, pp. 5198–5204, 2005. View at Publisher · View at Google Scholar · View at Scopus
  24. Y. Wan, H. Wu, A. Yu, and D. Wen, “Biodegradable polylactide/chitosan blend membranes,” Biomacromolecules, vol. 7, no. 4, pp. 1362–1372, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. X. Guan, X. Liu, and Z. Su, “Preparation and photophysical behaviors of fluorescent chitosan bearing fluorescein: potential biomaterial as temperature/pH probes,” Journal of Applied Polymer Science, vol. 104, no. 6, pp. 3960–3966, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. C. Cristallini, G. D. Guerra, N. Barbani, and F. Bianchi, “Biodegradable bioartificial materials made with chitosan and poly(vinyl alcohol). Part I: physicochemical characterization,” Journal of Applied Biomaterials and Biomechanics, vol. 5, no. 3, pp. 184–191, 2007. View at Google Scholar · View at Scopus
  27. C. Cristallini, N. Barbani, F. Bianchi, D. Silvestri, and G. D. Guerra, “Biodegradable bioartificial materials made by chitosan and poly(vinyl alcohol). Part II: enzymatic degradability and drug-releasing ability,” Biomedical Engineering Applications, Basis and Communications, vol. 20, no. 5, pp. 321–328, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. D. Silvestri, N. Barbani, G. D. Guerra, M. Gagliardi, and C. Cristallini, “Biodegradable bioartificial materials made by chitosan and poly(vinyl alcohol) part III: materials toughened by means of a dehydrothermal treatment,” Biomedical Engineering Applications, Basis and Communications, vol. 22, no. 6, pp. 509–517, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. S. Nguyen, S. Hisiger, M. Jolicoeur, F. M. Winnik, and M. D. Buschmann, “Fractionation and characterization of chitosan by analytical SEC and 1H NMR after semi-preparative SEC,” Carbohydrate Polymers, vol. 75, no. 4, pp. 636–645, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. D. Li, H. Yamamoto, H. Takeuchi, and Y. Kawashima, “A novel method for modifying AFM probe to investigate the interaction between biomaterial polymers (Chitosan-coated PLGA) and mucin film,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 75, no. 2, pp. 277–283, 2010. View at Publisher · View at Google Scholar · View at Scopus
  31. J. Fan and W. D. Yu, “Biomaterials from wool wastes: characterization of cortical cells/chitosan composite,” Waste Management and Research, vol. 28, no. 1, pp. 44–50, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Kocun, M. Grandbois, and L. A. Cuccia, “Single molecule atomic force microscopy and force spectroscopy of chitosan,” Colloids and Surfaces B: Biointerfaces, vol. 82, no. 2, pp. 470–476, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. H. Yang, R. Yuan, Y. Chai, and Y. Zhuo, “Electrochemically deposited nanocomposite of chitosan and carbon nanotubes for detection of human chorionic gonadotrophin,” Colloids and Surfaces B: Biointerfaces, vol. 82, no. 2, pp. 463–469, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. S. Tripathi, K. Rinki, P. K. Dutta, A. J. Hunt, D. J. Macquarrie, and J. H. Clark, “Direct chitosan scaffold formation via chitin whiskers by supercritical carbon dioxide method: a green approach,” in Proceedings of the 1st International Conference on Nanostructured Materials and Nanocomposites (ICNM '09), pp. 234–241, Applied Science Innovations Private Limited, Kottayam, India, 2009.
  35. R. A. A. Muzzarelli, “Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone,” Carbohydrate Polymers, vol. 76, no. 2, pp. 167–182, 2009. View at Publisher · View at Google Scholar · View at Scopus
  36. R. Muzzarelli, G. Biagini, A. Pugnaloni et al., “Reconstruction of parodontal tissue with chitosan,” Biomaterials, vol. 10, no. 9, pp. 598–603, 1989. View at Publisher · View at Google Scholar · View at Scopus
  37. R. A. A. Muzzarelli, G. Biagini, M. Bellardini, L. Simonelli, C. Castaldini, and G. Fratto, “Osteoconduction exerted by methylpyrrolidinone chitosan used in dental surgery,” Biomaterials, vol. 14, no. 1, pp. 39–43, 1993. View at Publisher · View at Google Scholar · View at Scopus
  38. R. A. A. Muzzarelli, M. Mattioli-Belmonte, C. Tietz et al., “Stimulatory effect on bone formation exerted by a modified chitosan,” Biomaterials, vol. 15, no. 13, pp. 1075–1081, 1994. View at Publisher · View at Google Scholar · View at Scopus
  39. N. Barbani, E. Rosellini, C. Cristallini, G. D. Guerra, A. Krajewski, and M. Mazzocchi, “Hydroxyapatite-collagen composites. Part I: can the decrease of the interactions between the two components be a physicochemical component of osteoporosis in aged bone?” Journal of Materials Science: Materials in Medicine, vol. 22, no. 3, pp. 637–646, 2011. View at Google Scholar
  40. R. A. A. Muzzarelli, G. Biagini, M. Mattioli Belmonte et al., “Osteoinduction by chitosan-complexed BMP: morpho-structural responses in an osteoporotic model,” Journal of Bioactive and Compatible Polymers, vol. 12, no. 4, pp. 321–329, 1997. View at Google Scholar · View at Scopus
  41. M. Mattioli Belmonte, A. De Benedittis, R. A. A. Muzzarelli et al., “Bioactivity modulation of bioactive materials in view of their application in osteoporotic patients,” Journal of Materials Science: Materials in Medicine, vol. 9, no. 9, pp. 485–492, 1998. View at Publisher · View at Google Scholar · View at Scopus
  42. R. A. A. Muzzarelli, V. Ramos, V. Stanic et al., “Osteogenesis promoted by calcium phosphate N,N-dicarboxymethyl chitosan,” Carbohydrate Polymers, vol. 36, no. 4, pp. 267–276, 1998. View at Google Scholar · View at Scopus
  43. M. Mattioli-Belmonte, N. Nicoli-Aldini, A. De Benedittis et al., “Morphological study of bone regeneration in the presence of 6-oxychitin,” Carbohydrate Polymers, vol. 40, no. 1, pp. 23–27, 1999. View at Publisher · View at Google Scholar · View at Scopus
  44. M. Mattioli Belmonte, A. De Benedittis, R. Mongiorgi et al., “Bioactivity of chitosan in dentistry. Preliminary data on chitosan-based cements,” Minerva Stomatologica, vol. 48, no. 12, pp. 567–576, 1999. View at Google Scholar · View at Scopus
  45. C. Muzzarelli and R. A. A. Muzzarelli, “Natural and artificial chitosan-inorganic composites,” Journal of Inorganic Biochemistry, vol. 92, no. 2, pp. 89–94, 2002. View at Publisher · View at Google Scholar · View at Scopus
  46. Y. Wan, Y. Fang, H. Wu, and X. Cao, “Porous polylactide/chitosan scaffolds for tissue engineering,” Journal of Biomedical Materials Research A, vol. 80, no. 4, pp. 776–789, 2007. View at Publisher · View at Google Scholar · View at Scopus
  47. H. Wu, Y. Wan, S. Dalai, and R. Zhang, “Response of rat osteoblasts to polycaprolactone/chitosan blend porous scaffolds,” Journal of Biomedical Materials Research A, vol. 92, no. 1, pp. 238–245, 2010. View at Publisher · View at Google Scholar · View at Scopus
  48. Q. L. Li, M. Y. Wu, L. L. Tang, J. Zhou, Y. Jiang, and B. W. Darvell, “Bioactivity of a novel nanocomposite of hydroxyapatite and chitosan-phosphorylated chitosan polyelectrolyte complex,” Journal of Bioactive and Compatible Polymers, vol. 23, no. 6, pp. 520–531, 2008. View at Publisher · View at Google Scholar · View at Scopus
  49. G. D. J. Rao, N. N. Balasubramanian, S. Prathaban, and B. J. William, “Clinical evaluation of chitin and chitosan in the management of fractures,” Tamilnadu Journal of Veterinary and Animal Sciences, vol. 5, no. 1, pp. 1–3, 2009. View at Google Scholar
  50. C. H. Chang, C. T. Tsao, K. Y. Chang et al., “Chitosan membrane with surface-bonded growth factor in guided tissue regeneration applications,” Journal of Bioactive and Compatible Polymers, vol. 25, no. 5, pp. 465–482, 2010. View at Publisher · View at Google Scholar · View at Scopus
  51. J. Venkatesan, Z. J. Qian, B. Ryu, N. A. Kumar, and S. K. Kim, “Preparation and characterization of carbon nanotube-grafted-chitosan—natural hydroxyapatite composite for bone tissue engineering,” Carbohydrate Polymers, vol. 83, no. 2, pp. 569–577, 2011. View at Publisher · View at Google Scholar · View at Scopus
  52. R. Nirmala, R. Navamathavan, H. S. Kang, M. H. El-Newehy, and H. Y. Kim, “Preparation of polyamide-6/chitosan composite nanofibers by a single solvent system via electrospinning for biomedical applications,” Colloids and Surfaces B, vol. 83, no. 1, pp. 173–178, 2011. View at Publisher · View at Google Scholar · View at Scopus
  53. S. C. C. C. Miranda, G. A. B. Silva, R. C. R. Hell, M. D. Martins, J. B. Alves, and A. M. Goes, “Three-dimensional culture of rat BMMSCs in a porous chitosan-gelatin scaffold: a promising association for bone tissue engineering in oral reconstruction,” Archives of Oral Biology, vol. 56, no. 1, pp. 1–15, 2011. View at Publisher · View at Google Scholar · View at Scopus
  54. R. A. A. Muzzarelli, “Chitosan composites with inorganics, morphogenetic proteins and stem cells, for bone regeneration,” Carbohydrate Polymers, vol. 83, no. 4, pp. 1433–1445, 2011. View at Publisher · View at Google Scholar · View at Scopus
  55. S. M. Barinov, V. S. Komlev, I. V. Fadeeva, A. Y. Fedotov, and A. S. Fomin, “Porous composite chitosan and gelatin-based material for filling of bone defects,” Russ., CODEN: RUXXE7 RU 2412711 C1 20110227 Application: RU 2009-134778 20090917. Priority: RU 2009-134778 20090917. Patent written in Russian. CAN 154:268927, 6 pages, 2011.
  56. R. Muzzarelli, V. Bicchiega, G. Biagini, A. Pugnaloni, and R. Rizzoli, “Role of N-Carboxybutyl chitosan in the repair of the meniscus,” Journal of Bioactive and Compatible Polymers, vol. 7, no. 2, pp. 130–148, 1992. View at Google Scholar · View at Scopus
  57. M. Mattioli-Belmonte, A. Gigante, R. A. A. Muzzarelli et al., “N,N-dicarboxymethyl chitosan as delivery agent for bone morphogenetic protein in the repair of articular cartilage,” Medical and Biological Engineering and Computing, vol. 37, no. 1, pp. 130–134, 1999. View at Google Scholar · View at Scopus
  58. C. D. Hoemann, J. Sun, A. Légaré, M. D. McKee, and M. D. Buschmann, “Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle,” Osteoarthritis and Cartilage, vol. 13, no. 4, pp. 318–329, 2005. View at Publisher · View at Google Scholar · View at Scopus
  59. S. R. Frenkel, G. Bradica, J. H. Brekke et al., “Regeneration of articular cartilage—evaluation of osteochondral defect repair in the rabbit using multiphasic implants,” Osteoarthritis and Cartilage, vol. 13, no. 9, pp. 798–807, 2005. View at Publisher · View at Google Scholar · View at Scopus
  60. C. D. Hoemann, J. Sun, M. D. McKee et al., “Chitosan-glycerol phosphate/blood implants elicit hyaline cartilage repair integrated with porous subchondral bone in microdrilled rabbit defects,” Osteoarthritis and Cartilage, vol. 15, no. 1, pp. 78–89, 2007. View at Publisher · View at Google Scholar · View at Scopus
  61. A. Chevrier, C. D. Hoemann, J. Sun, and M. D. Buschmann, “Chitosan-glycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects,” Osteoarthritis and Cartilage, vol. 15, no. 3, pp. 316–327, 2007. View at Publisher · View at Google Scholar · View at Scopus
  62. H. Wu, Y. Wan, X. Cao, and Q. Wu, “Proliferation of chondrocytes on porous poly(dl-lactide)/chitosan scaffolds,” Acta Biomaterialia, vol. 4, no. 1, pp. 76–87, 2008. View at Publisher · View at Google Scholar · View at Scopus
  63. H. J. Chung, J. W. Bae, H. D. Park, J. W. Lee, and K. D. Park, “Thermosensitive chitosans as novel injectable biomaterials,” Macromolecular Symposia, vol. 224, no. 1, pp. 275–286, 2005. View at Publisher · View at Google Scholar · View at Scopus
  64. K. M. Park, S. Y. Lee, Y. K. Joung, J. S. Na, M. C. Lee, and K. D. Park, “Thermosensitive chitosan-Pluronic hydrogel as an injectable cell delivery carrier for cartilage regeneration,” Acta Biomaterialia, vol. 5, no. 6, pp. 1956–1965, 2009. View at Publisher · View at Google Scholar · View at Scopus
  65. T. Hao, N. Wen, J. K. Cao et al., “The support of matrix accumulation and the promotion of sheep articular cartilage defects repair in vivo by chitosan hydrogels,” Osteoarthritis and Cartilage, vol. 18, no. 2, pp. 257–265, 2010. View at Publisher · View at Google Scholar · View at Scopus
  66. A. Chevrier, C. D. Hoemann, J. Sun, and M. D. Buschmann, “Temporal and spatial modulation of chondrogenic foci in subchondral microdrill holes by chitosan-glycerol phosphate/blood implants,” Osteoarthritis and Cartilage, vol. 19, no. 1, pp. 136–144, 2011. View at Publisher · View at Google Scholar · View at Scopus
  67. H. Gong, Y. Zhong, J. Li, Y. Gong, N. Zhao, and X. Zhang, “Studies on nerve cell affinity of chitosan-derived materials,” Journal of Biomedical Materials Research, vol. 52, no. 2, pp. 285–295, 2000. View at Publisher · View at Google Scholar · View at Scopus
  68. G. Lu, L. Kong, B. Sheng, G. Wang, Y. Gong, and X. Zhang, “Degradation of covalently cross-linked carboxymethyl chitosan and its potential application for peripheral nerve regeneration,” European Polymer Journal, vol. 43, no. 9, pp. 3807–3818, 2007. View at Publisher · View at Google Scholar · View at Scopus
  69. L. Zheng, Q. Ao, H. Han, X. Zhang, and Y. Gong, “Evaluation of the chitosan/glycerol-β-phosphate disodium salt hydrogel application in peripheral nerve regeneration,” Biomedical Materials, vol. 5, no. 3, Article ID 035003, 2010. View at Publisher · View at Google Scholar · View at Scopus
  70. I. Yamaguchi, S. Itoh, M. Suzuki, A. Osaka, and J. Tanaka, “The chitosan prepared from crab tendons: II. The chitosan/apatite composites and their application to nerve regeneration,” Biomaterials, vol. 24, no. 19, pp. 3285–3292, 2003. View at Publisher · View at Google Scholar · View at Scopus
  71. C. E. Schmidt and J. B. Leach, “Neural tissue engineering: strategies for repair and regeneration,” Annual Review of Biomedical Engineering, vol. 5, pp. 293–347, 2003. View at Publisher · View at Google Scholar · View at Scopus
  72. X. Wang, W. Hu, Y. Cao, J. Yao, J. Wu, and X. Gu, “Dog sciatic nerve regeneration across a 30-mm defect bridged by a chitosan/PGA artificial nerve graft,” Brain, vol. 128, no. 8, pp. 1897–1910, 2005. View at Publisher · View at Google Scholar · View at Scopus
  73. G. Ciardelli and V. Chiono, “Materials for peripheral nerve regeneration,” Macromolecular Bioscience, vol. 6, no. 1, pp. 13–26, 2006. View at Publisher · View at Google Scholar · View at Scopus
  74. M. Patel, P. J. VandeVord, H. W. Matthew, S. De Silva, B. Wu, and P. H. Wooley, “Collagen-chitosan nerve guides for peripheral nerve repair: a histomorphometric study,” Journal of Biomaterials Applications, vol. 23, no. 2, pp. 101–121, 2008. View at Publisher · View at Google Scholar · View at Scopus
  75. F. Xie, Q. F. Li, B. Gu, K. Liu, and G. X. Shen, “In vitro and in vivo evaluation of a biodegradable chitosan-PLA composite peripheral nerve guide conduit material,” Microsurgery, vol. 28, no. 6, pp. 471–479, 2008. View at Publisher · View at Google Scholar · View at Scopus
  76. Y. Cho, R. Shi, and R. B. Borgens, “Chitosan produces potent neuroprotection and physiological recovery following traumatic spinal cord injury,” Journal of Experimental Biology, vol. 213, no. 9, pp. 1513–1520, 2010. View at Publisher · View at Google Scholar · View at Scopus
  77. V. I. Scanga, A. Goraltchouk, N. Nussaiba, M. S. Shoichet, and C. M. Morshead, “Biomaterials for neural-tissue engineering—chitosan supports the survival, migration, and differentiation of adult-derived neural stem and progenitor cells,” Canadian Journal of Chemistry, vol. 88, no. 3, pp. 277–287, 2010. View at Publisher · View at Google Scholar · View at Scopus
  78. X. Lu, Z. Qiu, Y. Wan, Z. Hu, and Y. Zhao, “Preparation and characterization of conducting polycaprolactone/chitosan/ polypyrrole composites,” Composites A, vol. 41, no. 10, pp. 1516–1523, 2010. View at Publisher · View at Google Scholar · View at Scopus
  79. Y. Wan, J. Gao, J. Zhang, W. Peng, and G. Qiu, “Biodegradability of conducting chitosan-g-polycaprolactone/polypyrrole conduits,” Polymer Degradation and Stability, vol. 95, no. 10, pp. 1994–2002, 2010. View at Publisher · View at Google Scholar · View at Scopus
  80. C. Huang, R. Chen, Q. Ke, Y. Morsi, K. Zhang, and X. Mo, “Electrospun collagen-chitosan-TPU nanofibrous scaffolds for tissue engineered tubular grafts,” Colloids and Surfaces B: Biointerfaces, vol. 82, no. 2, pp. 307–315, 2011. View at Publisher · View at Google Scholar · View at Scopus
  81. A. Cooper, N. Bhattarai, and M. Zhang, “Fabrication and cellular compatibility of aligned chitosan-PCL fibers for nerve tissue regeneration,” Carbohydrate Polymers, vol. 85, no. 1, pp. 149–156, 2011. View at Publisher · View at Google Scholar
  82. Y. C. Kuo and C. F. Yeh, “Effect of surface-modified collagen on the adhesion, biocompatibility and differentiation of bone marrow stromal cells in poly(lactide-co-glycolide)/chitosan scaffolds,” Colloids and Surfaces B: Biointerfaces, vol. 82, no. 2, pp. 624–631, 2011. View at Publisher · View at Google Scholar · View at Scopus
  83. G. Ciapetti, L. Ambrosio, G. Marletta, N. Baldini, and A. Giunti, “Human bone marrow stromal cells: in vitro expansion and differentiation for bone engineering,” Biomaterials, vol. 27, no. 36, pp. 6150–6160, 2006. View at Publisher · View at Google Scholar · View at Scopus
  84. R. A. Weber, W. C. Breidenbach, R. E. Brown, M. E. Jabaley, and D. P. Mass, “A randomized prospective study of polyglycolic acid conduits for digital nerve reconstruction in humans,” Plastic and Reconstructive Surgery, vol. 106, no. 5, pp. 1036–1045, 2000. View at Google Scholar · View at Scopus
  85. V. Hung and A. L. Dellon, “Reconstruction of a 4 cm human median nerve gap by including an autogenous nerve slice in a bioabsorbable nerve conduit: case report,” Journal of Hand Surgery, vol. 33, no. 3, pp. 313–315, 2008. View at Publisher · View at Google Scholar · View at Scopus
  86. W. Fan, J. Gu, W. Hu et al., “Repairing a 35-MM-long median nerve defect with a chitosan/PGA artificial nerve graft in the human: a case study,” Microsurgery, vol. 28, no. 4, pp. 238–242, 2008. View at Publisher · View at Google Scholar · View at Scopus
  87. X. Gu, F. Ding, Y. Yang, and J. Liu, “Construction of tissue engineered nerve grafts and their application in peripheral nerve regeneration,” Progress in Neurobiology, vol. 93, no. 2, pp. 204–230, 2011. View at Publisher · View at Google Scholar · View at Scopus