Table of Contents
International Journal of Carbohydrate Chemistry
Volume 2011, Article ID 809743, 13 pages
Research Article

Consequences of Neutralization on the Proliferation and Cytoskeletal Organization of Chondrocytes on Chitosan-Based Matrices

Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, 207 Othmer Hall Lincoln, NE 68588-0643, USA

Received 2 February 2011; Revised 4 May 2011; Accepted 2 July 2011

Academic Editor: José das Neves

Copyright © 2011 Sandra E. Noriega and Anuradha Subramanian. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In tissue engineering strategies that seek to repair or regenerate native tissues, adhesion of cells to scaffolds or matrices is essential and has the potential to influence subsequent cellular events. Our focus in this paper is to better understand the impact of cellular seeding and adhesion in the context of cartilage tissue engineering. When scaffolds or surfaces are constructed from chitosan, the scaffolds must be first neutralized with sodium hydroxide and then washed copiously to render the surface, cell compatible. We seek to better understand the effect of surface pretreatment regimen on the cellular response to chitosan-based surfaces. In the present paper, sodium hydroxide concentration was varied between 0.1 M and 0.5 M and two different contacting times were studied: 10 minutes and 30 minutes. The different pretreatment conditions were noted to affect cell proliferation, morphology, and cytoskeletal distribution. An optimal set of experimental parameters were noted for improving cell growth on scaffolds.