143052.fig.004a
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143052.fig.004b
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143052.fig.004c
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Figure 4: Overexpression of -arrestins differentially influences MCHR1 internalization. BHK-570 cells were transfected with VSVg-MCHR1 and either arrestin-1 or arrestin-2. (a) Cells were treated with 1  M MCH or vehicle for 30 min and internalization was measured utilizing a cell-based ELISA. Data from Figure 3 was replotted. Ave ± SEM plotted. , . VSVg-MCHR1 and GFP -arrestin 1-expressing cells (b) and VSVg-MCHR1 and GFP -arrestin 2-expressing cells (c) were treated with 1  M MCH or vehicle for 30 min, then fixed, and immunostained with anti-VSVg antibody. Fluorescence microscopy was performed and overlay images compared.