Two Novel CYP11B1 Gene Mutations in Patients from Two Croatian Families with 11β-Hydroxylase Deficiency

Table 2

List of primers for PCR amplification and DNA sequencing of the CYP11B1 gene.

Primer sequence	Location	Orientation	Purpose
TCGAAGGCAAGGCACCAG	Promoter	sense	Exons 1-2 amplification
TGCTCCCAGCTCTCAGCT	Intron 2	antisense	Exons 1-2 amplification and Exon 1 sequencing
AGAAAATCCCTCCCCCCTA	Intron 2	sense	Exons 3–5 amplification
GACACGTGGGCGCCGTGTGA	Intron 5	antisense	Exons 3–5 amplification
TGACCCTGCAGCTGTGTCT	Intron 5	sense	Exons 6–9 amplification
GAGACGTGATTAGTTGATGGC	Exon 9, 3′ UTR	antisense	Exons 6–9 amplification
CACCAGGCAAGATAAAAG	Promoter	sense	Exon 1 sequencing
AGACACTTTGGATTGGGAC	Intron 1	sense	Exon 2 sequencing
AGGATGCACTGCTGAGCAC	Intron 3	antisense	Exon 3 sequencing
GTGGAGAGGGAGAAATTGGG	Intron 4	antisense	Exon 4 sequencing
AGGATGTTTCCCAGCACCAAAG	Intron 4	sense	Exon 5 sequencing
ATTCCAGAGGAAGAAGAGC	Intron 6	antisense	Exon 6 sequencing
CATGGATCTGGGACCTCTG	Intron 6	sense	Exon 7 sequencing
AGGCCAGTCCCACATTGCTC	Intron 8	antisense	Exon 8 sequencing
CCCCCTTCAGCATAATCTC	Intron 8	sense	Exon 9 sequencing

(Footnote) 3′ UTR: 3′ untranslated region.