Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent Mechanism

Figure 3

Western blot analysis of Wnt target genes protein expression (a) CD44, (b) TCF-1, (c) LEF-1ΔN, and (d) cMyc in Saos-2 osteoblastic cells grown as described in Figure 1. In each panel, upper row is the target gene and lower panel is loading control (βactin). Graph depicts protein quantification from Western blot evaluation. Bars represent mean ± SE of three different independent experiments performed in duplicate. ^∗𝑃<0.05 versus CTL; ^§𝑃<0.05 versus O.

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