Smooth Muscle-Specific BCL6+/− Knockout Abrogates Sex Bias in Chronic Hypoxia-Induced Pulmonary Arterial Hypertension in Mice

Figure 6

Abrogation of the male sex bias in chronic hypoxia-induced PAH in SMC:BCL6+/− mice: muscularization of pulmonary vessels in lung sections and right ventricular hypertrophy (Fulton’s index). (a) Lung sections from mice shown in Figure 4 were immunostained for SMA and the average number of muscularized vessels per 10x field were enumerated for each (mean ± SE). (b) Hearts derived from the experiment in Figure 4 were fixed in 4% formalin, rinsed in PBS and dissected into the right ventricle (RV) and the left ventricle plus septum (LV + S) portions. The ratio of the wet weights of RV/LV + S (mean ± SE) illustrates the extent of right ventricular hypertrophy irrespective of the total weight of the individual heart. by ANOVA; NS, not significant.

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