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International Journal of Endocrinology
Volume 2018, Article ID 7351902, 7 pages
https://doi.org/10.1155/2018/7351902
Research Article

Elevated and Correlated Expressions of miR-24, miR-30d, miR-146a, and SFRP-4 in Human Abdominal Adipose Tissue Play a Role in Adiposity and Insulin Resistance

1Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA
2University of Central Florida College of Medicine, Orlando, FL, USA
3Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA
4The Chemical Engineering Department, Massachusetts Institute of Technology, Cambridge, MA, USA

Correspondence should be addressed to Attila A. Seyhan; moc.oohay@nahyes_alitta

Received 14 October 2017; Accepted 6 December 2017; Published 12 March 2018

Academic Editor: Guido Sebastiani

Copyright © 2018 Yury O. Nunez Lopez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. We explored the relationships among microRNAs (miRNAs) and SFRP4, as they relate to adipose tissue functions including lipolysis, glucose and glycerol turnover, and insulin sensitivity. Methods. Abdominal adipose tissue (AbdAT) levels of thirteen microRNAs (miRNAs), SFRP4, and VEGF in lean nondiabetic subjects (), subjects with obesity (), and subjects with obesity and type 2 diabetes (T2DM) () were measured by qPCR. Insulin sensitivity was measured by the euglycemic-hyperinsulinemic clamp. Osmium fixation and Coulter counting were used for adipocyte sizing. Data were analyzed using generalized linear models that adjusted for age, gender, and ethnicity. Results. AbdAT miR-24, miR-30d, and miR-146a were elevated in subjects with obesity () and T2DM () and positively correlated with measures of percent body fat by DXA (, , ), and AbdAT SFRP4 (, , ). These three miRNAs additionally correlated among themselves (, , ). Conclusions. This study suggests a novel association between the elevated levels of miRNAs miR-24, miR-30d, and miR-146a (apparently coregulated) and the level of SFRP4 transcript in AbdAT of subjects with obesity and T2DM. These molecules might be part of a regulatory loop involved in AbdAT remodeling/adiposity and systemic insulin resistance. This trial is registered with NCT00704197.