Osteocalcin Alleviates Nonalcoholic Fatty Liver Disease in Mice through GPRC6A

Figure 3

The effects of osteocalcin and GPRC6ALKO on HFD-induced hepatic steatosis. H&E staining (×400) and Oil Red O staining (×200) were performed in the liver tissues: (a), (e) WT : HFD + NS; (b), (f) WT : HFD + OCN; (c), (g) GPRC6ALKO : HFD + NS; (d), (h) GPRC6ALKO : HFD + OCN; (i) liver content of TGs. In Figure 3(i), data were expressed as the mean ± SEM and were analyzed by independent-samples T-test. , the WT : HFD + OCN group compared with the WT : HFD + NS group. ^&, the GPRC6ALKO : HFD + OCN group compared with the WT : HFD + OCN group. GPRC6ALKO, liver-specific conditional GPRC6A knockout; WT, wild-type; NS, saline solution; HFD, high-fat diet; OCN, osteocalcin.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)