Characteristics and Antioxidant Activity of Fucoidan from Sargassum hystrix: Effect of Extraction Method

Table 2

Effect of the extraction method on the antioxidant activity derived from the fucoidan from Sargassum hystrix (DPPH, FRAP, HRSA, and total antioxidants).

Extraction methods	Antioxidant activity
	DPPH (IC50, ppm)	FRAP (μM/g)	HRSA (IC50, ppm)	Total antioxidant (μg AAE/mg)
A	^b	^a	^a	^b
B	^bc	^bc	^b	^b
C	^bc	^b	^c	^a
D	^c	^bc	^a	^a
Cf	^c	^c	^b	^a

Note: A (0.1 N HCl, room temperature, 24 h), B (2% CaCl₂, 85°C, 4 h), C (85% ethanol, room temperature, 12 h), D (0.5% EDTA, 70°C, 3 h), and Cf (commercial fucoidan). ^a-cThe same letter in the same column shows an insignificant difference ().