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International Journal of Genomics
Volume 2014, Article ID 483905, 10 pages
http://dx.doi.org/10.1155/2014/483905
Research Article

In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae

1Department of Allergy, First Affiliated Hospital of Anhui Medical University, No. 210, Jixi Road, Anhui Province, Hefei 230022, China
2Department of Urology, First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, China
3Research Division of Clinical Pharmacology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China

Received 9 January 2014; Revised 28 March 2014; Accepted 30 March 2014; Published 8 May 2014

Academic Editor: Huai-Rong Luo

Copyright © 2014 Xiaohong Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The house dust mites are major sources of indoor allergens for humans, which induce asthma, rhinitis, dermatitis, and other allergic diseases. Der f 25 is a triosephosphate isomerase, representing the major allergen identified in Dermatophagoides farinae. The objective of this study was to predict the B and T cell epitopes of Der f 25. In the present study, we analyzed the physiochemical properties, function motifs and domains, and structural-based detailed features of Der f 25 and predicted the B cell linear epitopes of Der f 25 by DNAStar protean system, BPAP, and BepiPred 1.0 server and the T cell epitopes by NetMHCIIpan-3.0 and NetMHCII-2.2. As a result, the sequence and structure analysis identified that Der f 25 belongs to the triosephosphate isomerase family and exhibited a triosephosphate isomerase pattern (PS001371). Eight B cell epitopes (11–18, 30–35, 71–77, 99–107, 132–138, 173–187, 193–197, and 211–224) and five T cell epitopes including 26–34, 38–54, 66–74, 142–151, and 239–247 were predicted in this study. These results can be used to benefit allergen immunotherapies and reduce the frequency of mite allergic reactions.