Mutations in Homocysteine Metabolism Genes Increase Keratin N-Homocysteinylation and Damage in Mice

Figure 2

SDS-PAGE analysis of hair keratin from Cbs^−/− and Cbs^+/+ mice. Keratin was extracted from mouse hair (stored at 25°C for 6 months) with SDS/DTT solution [18], and the extracts were analyzed on 10% SDS-PAGE gels. Lanes 1–4, keratin from Cbs^−/− mice; lanes 5–8, keratin from their Cbs^+/+ littermates. Quantification by densitometry is shown in Table 4. The last lane on the right shows molecular weight markers.