Statin Modulation of Human T-Cell Proliferation, IL-1 and IL-17 Production, and IFN- T Cell Expression: Synergy with Conventional Immunosuppressive Agents
Figure 5
Percentage of IFN- expression in PMA/ionomycin stimulated whole blood with or without addition of drugs. Each bar represents the means ± SEM from 6 experiments, each performed in triplicate, subtracting basal cytokine expression. Drug concentrations: atorvastatin (50 μM), lovastatin (50 μM), simvastatin (100 μM), rapamycin (100 μM), mycophenolate (100 μM), CsA (100 ng/mL), and dexamethasone (400 μg/mL). *; **. One representative FACS analysis is shown. (A), Unstimulated CD3+ T cells showing basal IFN- expression. (B), Stimulated CD3+ T cells to demonstrate baseline levels of IFN- expression. (C), Stimulated CD3+ T cells treated with atorvastatin (50 μM) demonstrating a decreased level of IFN- expression as compared to control. (D), Stimulated CD3+ T cells treated with dexamethasone (400 µg/mL), showing a decreased level of IFN- as compared to group (C).
