Transcriptional Regulation of the rsbV Promoter Controlling Stress Responses to Ethanol, Carbon Limitation, and Phosphorous Limitation in Bacillus subtilis

Table 1

Bacillus subtilis strains used in this study.

Strain	Relevant genotype	Source or reference^(a)
JH642	trpC2 pheA1	BGSC
ST124	trpC2 pheA1 ccpA::Tn917	[2]
ST106	trpC2 pheA1 Δ(bgalX) amyE::(gntRK ′-lacZ) Δspo0E ptsK::Spc	Laboratory stock
SF416T	trpC2 amyE::(amtB-lacZ)416 neo tnrA62::Tn917erm	Susan Fisher
BCS50	trpC2 pheA1 amyE::rsbV1-lacZ	pDG-rsbV1 tf JH642
BCS52	trpC2 pheA1 amyE::rsbV1-lacZ ccpA::Tn917	ST124 tf BCS50
BCS54	trpC2 pheA1 amyE::rsbV1m1-lacZ	pDG-rsbV1m1 tf JH642
BCS55	trpC2 pheA1 amyE::rsbV3-lacZ	pDG-rsbV3 tf JH642
BCS56	trpC2 pheA1 amyE::rsbV5-lacZ	pDG-rsbV5 tf JH642
BCS58	trpC2 pheA1 amyE::rsbV1-lacZ ptsK::Spc	ST106 tf BCS50
BCS61	trpC2 pheA1 amyE::ctc-lacZ	pDG-ctc tf JH642
BCS62	trpC2 pheA1 amyE::ctc-lacZ ccpA::Tn917	ST124 tf BCS61
BCS68	trpC2 pheA1 amyE::rsbV1-lacZ tnrA62::Tn917erm	SF416T tf BCS50
BCS69	trpC2 pheA1 amyE::rsbV1-lacZ tnrA62::Tn917erm ccpA::Tn917	ST124 tf BCS68

^(a)tf, the indicated chromosomal DNA or plasmid was used to transform the indicated recipient strain.