New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA

Table 1

Characteristics of three patients with Mycoplasma hominis extra-genital infections, including clinical presentation and results of the real-time PCR.

Patient no.	Age	Sex	Clinical infection	Underlying conditions	Other etiology	Clinical specimen	M. hominis qPCR results in copies/mL	Other positive diagnostic tests
1	54	Female	Inguinal lymphadenitis with abscess formation	Neutropenia, HIV	None	Abscess fluid a	16,175,000	Specific culturec and broad-spectrum PCR
						Cervix swab	4,400
						Vaginal swab	130
						Urine	82
2	15	Female	Pneumonia and pericarditis	None	None	Abscess fluid a	98,500,000	Specific culturec and broad-spectrum PCR
						Pleural fluid	380,000
						Pericardiac fluid	85
						Sputumb	6
						Pleural fluidb	0
3	56	Male	Mediastinitis	Type A aortic dissection	None	Mediastinal fluid a	7,000	Blood agar d
						Mediastinal swab	460
						Blood	5

a First samples documenting the infection;
b Samples taken a few days after treatment start;
cCulture media: Mycoplasma IST, bioMérieux, France;
d Conventional culture positive on blood agar plate followed by sequencing for identification [13].