Aromatic Compound-Dependent Staphylococcus aureus Is Safe in a Nasal Colonization Leukopenic Murine Model

Table 1

Bacterial strains and plasmids used in this study.

Strain or plasmid	Comment	Source
RN4220	Mutant strain of Staph. aureus 8325-4 that accepts foreign DNA
RN6390	𝑎𝑔𝑟^+ laboratory strain related to 8325-4, maintains haemolytic pattern when propagated on sheep
	erythrocytes
Newman	S. aureus polysaccharide capsular serotype 5 (CP5)
NK41	Newman aroA::Ka^R
RD17; RD89	RN6390 ΔaroA	This study
DH5α	E. coli host cloning vectors	Invitrogen
pMAD	E. coli—S.aureus (Listeria) shuttle vector with the bgaB gene encoding a β-galactosidase. Amp^R/Em^R
pMAD-DEL	pMAD plasmid containing the mutant allele for deletion of the aroA gene	This study
pGEM-T Easy	Amp^R, 𝑙𝑎𝑐𝑍^′, f1 ori, MCS, Mob^−, cloning T vector	Promega
pALC1743	pSK236 (𝑔𝑓𝑝𝑢𝑣𝑟with agr P3 promoter)