Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field

Table 1

Differential characteristics that differentiate strain AK-1^T along with the closest species AK-1^T (MTCC 10524^T), A. aurantiacus (MTCC 11069^T), A. soli (MTCC 11074^T), A. tropicus (MTCC 11075^T), A. ulmi (MTCC 10783^T), A. flavus (MTCC 11103^T), and A. italicus (MTCC 10784^T).

Characteristics	AK-1^T (MTCC 10524^T)	A. aurantiacus (MTCC 11069^T)	A. soli (MTCC 11074^T)	A. tropicus (MTCC 11075^T)	A. ulmi (MTCC 10783^T)	A. flavus (MTCC 11103^T)	A. italicus (MTCC 10784^T)
Growth at
37°C	+	+	+	+	−	+	+
42°C	−	−	+	−	−	−	−
2% NaCl	−	−	+	+	−	−	+
5% NaCl	−	−	+	−	−	−	+
pH 5.0	+	−	−	+	+	+	+
pH 10.0	−	+	+	+	−	−	+
pH 12.0	−	−	+	+	−	−	+
Starch hydrolysis	+	+	−	+	−	+	−
Casein hydrolysis	−	−	−	−	−	+	−
Urease	−	+	−	−	−	−	−
Catalase	−	+	+	+	−	+	−
Acid production from carbohydrates
Salicin	−	+	+	+	+	−	+
Mannitol	−	−	−	+	+	+	−
Melibiose	−	−	−	+	−	−	−
Galactose	−	−	+	+	+	−	+
Arabinose	+	−	−	+	−	+	−
Cellobiose	+	−	+	+	+	−	+
Sucrose	−	+	+	+	+	−	+
Xylose	+	−	−	+	+	+	−
Inositol	−	−	−	−	−	+	−
Rhamnose	−	−	+	+	+	−	+
Lactose	+	+	−	+	−	−	−
Trehalose	+	+	+	+	+	−	−
Mannose	−	+	+	+	+	+	+
Maltose	−	−	+	+	+	−	+
Raffinose	−	+	+	+	+	−	+
Sensitivity to antibiotics (µg/disc)
Nitrofurantoin (300)	S	R	R	S	S	S	S
Norfloxacin (10)	R	S	R	R	R	S	R
Polymyxin B (300)	S	S	R	S	S	S	R
Kanamycin (30)	S	S	R	S	S	S	R
Colistin (10)	R	R	R	S	R	S	S
Methicillin (5)	R	S	R	S	R	S	S
Oxacillin (5)	R	S	R	S	R	S	S
Gentamycin (10)	S	R	S	S	S	S	S
Trimethoprim (5)	S	S	R	S	S	S	S
Oxytetracycline (30)	S	S	R	S	S	S	R
Cefoxitin (30)	S	S	R	S	R	S	S
Biochemical tests using VITEK 2GP card
Arginine dihydrolase 1	−	−	−	−	−	−	+
Leucine arylamidase	+	+	+	+	+	+	+
𝛼-Glucosidase	+	+	+	+	−	−	+
L-Proline arylamidase	+	+	+	+	−	+	+
𝛼-Galactosidase	−	+	−	+	−	−	−
Alanine arylamidase	+	+	+	+	−	+	+
Tyrosine arylamidase	−	+	+	−	−	+	+
L-Lactate alkalinization	−	−	−	−	−	−	+
Salicin	−	−	−	−	−	−	+
Quinone type	MK12; 11, 13	MK12, 13	MH12	MK12	M12, 11, 10	MK12	MK12, 13
Total lipid pattern	DPG, PG	DPG, PG	DPG, PG	DPG, PG	DPG, PG	DPG, PG	DPG, PG
DNA G + C mol%	71.8	72.8	73.4	72.7	72.0	70.9	70.8

All the strains were positive at pH 8.0 and 9.0, at temperatures 25°C and 30°C, and acid production from fructose; negative at 12°C, 10%, 15% NaCl, dulcitol, inositol, sorbitol, adonitol, citrate, methyl-red, Voges-Proskauer, indole, nitrate, and gelatin liquefaction. All the strains are negative for the following biochemical tests using VITEK 2-GP card: D-Amygdalin, phosphatidylinositol phospholipase C, D-xylose, 𝛽-galactosidase, Ala-Phe-Pro-arylamidase, cyclodextrin, L-aspartate arylamidase, 𝛽-galactopyranosidase, 𝛼-mannosidase, phosphatase, 𝛽-glucuronidase, L-pyrrolldonyl arylamidase, D-sorbitol, urease, polymyxin B resistance, D-galactose, D-ribose, lactose, N-acetyl-D-glucosamine, D-maltose, bacitracin resistance, novobiocin resistance, growth in 6.5% NaCl, D-mannitol, D-mannose, methyl-𝛽-D-glucopyranoside, pullulan, D-raffinose, O/129 resistance (comp. vibrio.), sucrose, D-trehalose, arginine dihydrolase 2 and optochin resistance. All the strains were sensitive to triple sulphas, kanamycin, sulfonamide, novobiocin, ampicillin, and rifampicin. S: sensitive; R: resistance.