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International Journal of Nephrology
Volume 2012, Article ID 154397, 7 pages
http://dx.doi.org/10.1155/2012/154397
Clinical Study

Transcription of the Tumor Suppressor Genes p53 and RB in Lymphocytes from Patients with Chronic Kidney Disease: Evidence of Molecular Senescence?

1Department of Medical Biopathology and Clinical Microbiology, Aeginition Hospital, Medical School, University of Athens, 115 28 Athens, Greece
2Department of Nephrology, General Hospital of Athens, “G. Gennimatas”, 115 27 Athens, Greece
3Department of Nursing, Sparta General Hospital Building Complex, Faculty of Human Movement and Quality of Life Sciences, University of Peloponnese, 23 100 Sparta, Greece
4Department of Statistics and Insurance Science, University of Piraeus, 185 34 Piraeus, Greece

Received 7 June 2012; Revised 18 August 2012; Accepted 21 August 2012

Academic Editor: Alejandro Martín-Malo

Copyright © 2012 Vasileios Kordinas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Patients suffering from renal failure exhibit an impaired immune system function. We wanted to investigate the transcription of the tumor suppressor genes p53 and RB to record, if these cells could be stimulated in vitro in order to divide, after the addition of antigenic and inflammatory factors. This expression was measured by real-time PCR in peripheral blood mononuclear cells (PBMCs) from three different groups: ten healthy individuals, ten patients with chronic kidney disease (CKD), and ten dialysis patients with end stage renal disease (ESRD). The transcription rate of these genes was also measured after the cultivation of PBMCs under four different conditions: just with the culture medium, with lipopolysaccharide (LPS), with C-reactive protein (CRP), and with lipoxin A4 (LXA4)-LPS. Our results show that in most cases after the cultivation with additives, the transcription levels were higher in dialysis patients compared to those of the other two groups. Our findings serve as indications of cellular senescence on a molecular level, while it seems that these cells are less easily stimulated in vitro in order to duplicate.