Morphological Retrospective Study of Peritoneal Biopsies from Patients with Encapsulating Peritoneal Sclerosis: Underestimated Role of Adipocytes as New Fibroblasts Lineage?

Figure 4

Representative photomicrographs demonstrating histopathological data of interstitial inflammatory cells infiltration in parietal peritoneal tissues biopsy using immunostaining of CD8 (a–d), CD4 (e–h), CD20 (i–l), and CD68 (m–p). Positive control of immunostainings for used antibodies (internal controls) (a, e, i, and m), control: normal peritoneum (b, f, j, and n), case of acute peritonitis (control 3; c, g, k, and o), and case  1 of encapsulating peritoneal sclerosis (EPS) (d, h, l, and p). Absence of mononuclear cells in normal peritoneum. Weak expression of all CD8, CD4, CD20, and CD68 in conjunctive tissue areas adjacent to mesothelium in case with acute peritonitis characterized mainly by polymorphonuclear cells infiltration. Marked interstitial inflammatory cells identified (d) several CD8+ cells (T lymphocytes), (h) few CD4+ cells (T lymphocytes), and (l) CD20+ (B cells), which were accompanied by several (f) CD68+ cells (macrophages) diffusely infiltrating fibrotic areas. Immunoperoxidase staining counterstained with hematoxylin. Original magnification: (a–p) ×20. Small pictures (a, e, i, and m): ×40.