Morphological Retrospective Study of Peritoneal Biopsies from Patients with Encapsulating Peritoneal Sclerosis: Underestimated Role of Adipocytes as New Fibroblasts Lineage?

Table 3

Results of immunohistochemical quantifications of mesothelio-mesenchymal transdifferentiation process, evaluation of interstitial inflammation, and vasculature in peritoneal biopsies samples from controls, case of acute peritonitis, and patients with encapsulating peritoneal sclerosis.

	Control 1	Control 2	Case of acute peritonitis	Case EPS 1	Case EPS 2	Case EPS 3
Markers of mesothelial cells phenotype
AE1/AE3
Mesothelium	(+++)	(+++)	(+++)	0	(0)	0
Interstitium	0	0	0	(+++)	(+++)	(+++)
Calretinin
Mesothelium	(+++)	(+++)	(+++)	0	0	0
Interstitium	0	0	(+/−)	(++)	(+)	(+)
Immunohistochemical phenotyping of inflammatory infiltrating cells
CD4	19.6	11.5	8.85	1.05	10.8	ND
CD8	1.15	0	1.05	0.9	2.25	130
CD68	0.9	1.25	4.7	2.75	1.66	18.8
CD20	3	0	0	3	10	1
Markers of endothelial cells phenotype
CD31^#	0.95	0.8	4.2	6.5	0.5	ND
Markers of mesenchymal cells phenotype
Vimentin
Mesothelium	0	0	(+)	(+++)	(+++)	(+)
Interstitium	0	0	(+)	(+++)	(+++)	(+++)
Markers of myofibroblasts
Alpha SMA	0	0	(+)	(+++)	(+++)	(+++)

EPS: encapsulating peritoneal sclerosis; SMA: alpha-smooth muscle actin. ND: no data available (insufficiency of biopsy material). Semiquantitative score analysis: expression; high: (+++), moderate: (++), low: (+), and absent: 0. Score of quantitative evaluations: () mean number of cells or (#) vessels per field (details of all immunostaining analysis are described in Material and Methods).