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International Journal of Vascular Medicine
Volume 2012, Article ID 127149, 7 pages
http://dx.doi.org/10.1155/2012/127149
Clinical Study

Expression and Function of Ephrin-B1 and Its Cognate Receptor EphB2 in Human Abdominal Aortic Aneurysm

1Department of Vascular Physiology, National Cerebral and Cardiovascular Center, Suita, Osaka 565-8565, Japan
2Division of Cardiovascular Medicine, Kanazawa University Graduate School of Medicine, Kanazawa, Ishikawa 920-8640, Japan
3Department of Pathology, National Cerebral and Cardiovascular Center, Suita, Osaka 565-8565, Japan
4Cardiovascular Surgery, National Cerebral and Cardiovascular Center, Suita, Osaka 565-8565, Japan

Received 29 March 2012; Revised 22 May 2012; Accepted 6 June 2012

Academic Editor: Muredach P. Reilly

Copyright © 2012 Aiji Sakamoto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We examined the expression of ephrin-B1 and its cognate receptor EphB2, key regulators of angiogenesis and embryogenesis, in human abdominal aortic aneurysm (AAA) and analyzed their functional roles in cell migration. From 10 patients (9 males and 1 female; age, 6 8 . 5 ± 2 . 4 ) who underwent vascular surgery for AAA, we obtained AAA and adjacent control tissues. Using real-time RT-PCR, we analyzed expression of ephrin-B1 and EphB2. We also histologically localized these molecules in AAA tissues. Finally, effects of ephrin-B1 and EphB2 on inflammatory cell chemotaxis were examined by cell migration assay. Expression levels of ephrin-B1 ( 0 . 4 1 0 ± 0 . 0 4 6 versus 1 . 1 9 8 ± 0 . 2 5 2 , 𝑃 = 0 . 0 2 7 ) and EphB2 ( 0 . 7 6 4 ± 0 . 2 1 2 versus 1 . 2 7 2 ± 0 . 1 3 7 , 𝑃 = 0 . 5 9 4 ) were higher in AAA than normal control. Both ephrin-B1 and EphB2 were expressed in macrophages, T lymphocytes, and endothelial cells within AAA. In chemotaxis assay, ephrin-B1 and EphB2 inhibited mononuclear-cell chemotaxis induced by stromal derived factor-1 down to 5 4 . 7 ± 1 2 . 7 % ( 𝑃 = 0 . 0 1 ) and 5 0 . 7 ± 1 3 . 1 % ( 𝑃 = 0 . 0 1 ), respectively. These data suggest that ephrin-B1 and EphB2 might be functional in human adult inflammatory cells and involved in the pathogenesis of AAA, although specific roles of these molecules should further be sought.