Table 1: Oligonucleotide primers used for extended spectrum β-lactamase and virulence gene detection.

Gene nameTypePrimer SequenceAnnealing temp.Amplicon size (bp)Reference

SHVFw5′-ACTATCGCCAGCAGGATC-3′53°C356This study
Rev5′-ATCGTCCACCATCCACTG-3′
TEMFw5′-GATCTCAACAGCGGTAAG-3′50°C786This study
Rev5′-CAGTGAGGCACCTATCTC-3′
CTX-M-15Fw5′-GTGATACCACTTCACCTC-3′49°C255This study
Rev5′-AGTAAGTGACCAGAATCAG-3′
TraTFw5′-GGTGTGGTGCGATGAGCACAG-3′63°C290 [23]
Rev5′-CACGGTTCAGCCATCCCTGAG-3′
FimHFw5′-TACTGCTGATGGGCTGGTC-3′50°C640 [24]
Rev5′-GCCGGAGAGGTAATACCCC-3′
IssFw5′-GGCAATGCTTATTACAGGATGTGC-3′50°C260 [24]
Rev5′-GAGCAATATACCCGGGCTTCC-3′
BssSFw5′-GATTCAATTTTGGCGATTCCTGC-3′48°C225 [24]
Rev5′-TAATGAAGTCATTCAGACTCATCC-3′
iucAFw5′-CGAAATCGAAATAGATCACC-3′51°C1125 [24]
Rev5′-CTGACGCGATTTGCCGC-3′

Forward, reverse, and base pair.