Review Article | Open Access
Ruža Pandel, Borut Poljšak, Aleksandar Godic, Raja Dahmane, "Skin Photoaging and the Role of Antioxidants in Its Prevention", International Scholarly Research Notices, vol. 2013, Article ID 930164, 11 pages, 2013. https://doi.org/10.1155/2013/930164
Skin Photoaging and the Role of Antioxidants in Its Prevention
Photoaging of the skin depends primarily on the degree of ultraviolet radiation (UVR) and on an amount of melanin in the skin (skin phototype). In addition to direct or indirect DNA damage, UVR activates cell surface receptors of keratinocytes and fibroblasts in the skin, which leads to a breakdown of collagen in the extracellular matrix and a shutdown of new collagen synthesis. It is hypothesized that dermal collagen breakdown is followed by imperfect repair that yields a deficit in the structural integrity of the skin, formation of a solar scar, and ultimately clinically visible skin atrophy and wrinkles. Many studies confirmed that acute exposure of human skin to UVR leads to oxidation of cellular biomolecules that could be prevented by prior antioxidant treatment and to depletion of endogenous antioxidants. Skin has a network of all major endogenous enzymatic and nonenzymatic protective antioxidants, but their role in protecting cells against oxidative damage generated by UV radiation has not been elucidated. It seems that skin’s antioxidative defence is also influenced by vitamins and nutritive factors and that combination of different antioxidants simultaneously provides synergistic effect.
Unlike chronological aging, which is predetermined by individual’s physiological predisposition, photoaging depends primarily on the degree of sun exposure and on an amount of melanin in the skin. Individuals who have a history of intensive sun exposure, live in sunny geographical areas, and have fair skin will experience the greatest amount of ultraviolet radiation (UVR) skin load and consequently severe photoaging [1, 2]. Clinical signs of photoaging include wrinkles, mottled pigmentation (hypo- or hyperpigmentation), rough skin, loss of the skin tone, dryness, sallowness, deep furrows, severe atrophy, telangiectasias, laxity, leathery appearance, solar elastosis, actinic purpura, precancerous lesions, skin cancer, and melanoma [3, 4]. Sun-exposed areas of the skin, such as the face, neck, upper chest, hands, and forearms, are the sites where these changes occur most often . Chronological skin aging, on the other hand, is characterized by laxity and fine wrinkles, as well as development of benign growths such as seborrheic keratoses and angiomas, but it is not associated with increased/decreased pigmentation or with deep wrinkles that are characteristic for photoaging . Seborrheic keratoses are regarded as best biomarker of intrinsic skin aging since thier appearance is independent on sun exposure. While intrinsically aged skin does not show vascular damage, photodamaged skin does. Studies in humans and in the albino and hairless mice showed that acute and chronic UVB irradiation greatly increases skin vascularization and angiogenesis [7, 8]. The sun is the main source of UVR and the main contributor to the photoaging. UVC radiation (100 to 290 nm) is almost completely absorbed by the ozone layer and does not affect the skin. UVB (290 to 320 nm) affects the superficial layer of the skin (epidermis) and causes sunburns. It is the most intense between 10 am and 2 pm, during summer months, does not penetrate through the glass, and accounts for 70% of a person’s yearly average cumulative UVB dose. UVA (320 to 400 nm) was believed to have a minor effect on the skin, but studies showed that they penetrate deeper in the skin (e.g., about 20% at 365 nm), are more abundant in sunlight (95% of UVA and 5% of UVB), and therefore exhibit more severe damage [9, 10]. Significantly more photons in the UVA are needed to cause the same degree of damage compared to UVB since they are less energetic, but they are present in much higher quantities in sunlight and are more penetrant than in UVB . Until recently, it has become evident that also infrared radiation (IR) could induce skin damage and contribute to the skin photoaging. While proton energy of IR is low, total amount of IR which reaches humans’ skin accounts approximately for 54% (compared to 5–7% of UV rays). Most of the IR lies within the IR-A band ( to 1440 nm), which represents approximately 30% of total solar energy, and penetrates human skin deeply compared to IR-B and IR-C, which only penetrate the upper skin layers. In comparison, IR-A penetrates the skin deeper than UV, and approximately 50% of it reaches the dermis. Molecular mechanisms of damaging effect of IR-A on the skin are attributed to induction of matrix mtalloproteinase-1, as well as to generation of reactive oxygen species (ROS). The exposure of human to environmental and artificial UVR has increased significantly in the last 50 years. This is due to an increased solar UVR as a consequence of the stratospheric ozone depletion, use of sunscreens, false believe of being well protected while exposed to sun for longer time, outdoor leisure activities, and prolonged life expectancy in industrialized countries .
2. Effects of UVR on Cells and Tissues
Studies in hairless mice demonstrated the carcinogenicity of UVR, with UVB being the most effective, followed by UVC and UVA . UVB radiation is three to four orders of magnitude more effective than UVA. In none of the experiments it was possible to exclude completely a contribution of UVC, but the size of the effects observed indicate that they cannot be due to UVB alone . People with a poor ability to tan, who burn easily, and have light eye and hair colour are at a higher risk of developing melanoma, basal-cell, and squamous-cell carcinomas. UVB most commonly causes cyclobutane pyrimidine dimmers. UVA, on the other hand, primarily causes DNA damage indirectly by the production of short-lived reactive oxygen species (ROS) such as singlet oxygen, superoxide, and H2O2 via endogenous photosensitizers. UVA radiation generates more phosphodiester bond breaks in DNA than would be expected by the total amount of energy directly absorbed by the DNA; therefore, it most likely causes indirect damage to DNA, which is caused by endogenous photosensitizers such as riboflavin, nicotinamide coenzymes, and rarely RNA bases . Damage of the skin cells’ DNA is repaired by two different mechanisms: nucleotide excision repair (NER) and base excision repair (BER). The ROS-induced DNA damage is primarily repaired by the BER system and damage caused by direct influence of UVR on DNA by the NER system. DNA damage that can be induced by UVA radiation includes pyrimidine dimmers, single-strand breaks (both are critical in UVA radiation-induced cellular lethality), and perhaps more importantly DNA protein cross-links [14–17]. On the other hand, ROS can oxidize guanine in DNA to form 8-hydroxy-7,8-dihydroguanine (8-OHdG). The frequency of this characteristic mutation in human skin increases with cumulative sun exposure and could be used as an internal marker of cumulative sun exposure . OH• can be added to guanine at positions 4, 5, and 8 (causing 8-OHdG) or undergoes opening of the imidazole ring, followed by one-electron reduction and protonation, to give 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FAPyG) . Photoexcitation of cytosine and guanine may lead to the formation of relatively rare 6-hydroxy-5,6-dihydrocytosine and 8-oxo-7,8-dihydroguanine.
A second mechanism, which requires participation of endogenous photosensitizers and oxygen, causes most of the DNA damage generated by the UVA and visible light. Singlet oxygen is likely to be mostly involved in the formation of 8-oxo-7,8-dihydroguanine that was observed within both isolated and cellular DNA. It may be expected that oxidized purine together with DNA strand breaks and pyrimidine base oxidation products is also generated with a lower efficiency through Fenton type reactions . The number of different DNA modifications that are capable of producing OH• appears to be over 100 .
Solar UVR induces a variety of photoproducts in DNA, including cyclobutane-type pyrimidine dimers, pyrimidine-pyrimidone (6-4) photoproducts, thymine glycols, cytosine damage, purine damage, DNA strand breaks, and DNA-protein cross links . Substantial information on biological consequences is available only for the first two classes. Both are potentially cytotoxic and can lead to mutations in cultured cells, and there is evidence that cyclobutane-type pyrimidine dimers may be precarcinogenic lesions .
UVR also directly or indirectly initiates and activates a complex cascade of biochemical reactions in the human skin. Besides, the UV light-induced ROS interfere with signalling pathways. On a molecular level, UVR activates cell surface receptors of keratinocytes and fibroblasts in the skin, which initiates signal transduction cascades. This, in turn, leads to a variety of molecular changes, which causes a breakdown of collagen in the extracellular matrix and a shutdown of new collagen synthesis . UV-induced liberation of ROS in human skin is responsible for stimulation of numerous signal transduction pathways via activation of cell surface cytokine and growth factor receptors. UVA or UVB induce activation (sometimes via peroxides or singlet O2 as signalling molecules) of a wide range of transcription factors in skin cells, including factor activator protein-1 (AP-1) . This can increase production of matrix metalloproteinases that can degrade collagen and other connective tissue components. For example, the UV light-induced ROS induce the transcription of AP-1. AP-1 induces upregulation of matrix metalloproteinases (MMPs) like collagenase-1 (MMP-1), stromelysin-1 (MMP-3), and gelatinase A (MMP-2), which specifically degrade connective tissue such as collagen and elastin and indirectly inhibit the collagen synthesis in the skin . As indicated by their name, these zinc-dependent endopeptidases show proteolytic activity in their ability to degrade matrix proteins such as collagen and elastin . Destruction of collagen is a hallmark of photoaging. The major enzyme responsible for collagen 1 digestion is matrix metalloproteinase-1 (MMP-1) . Skin fibroblasts produce MMP-1 in response to UVB irradiation, and keratinocytes play a major role through an indirect paracrine mechanism involving the release of epidermal cytokine after UVB irradiation . MMPs are produced in response to UVB irradiation in vivo and are considered to be involved in the changes in connective tissue that occur in photoaging . They are associated with a variety of normal and pathological conditions that involve degradation and remodelling of the matrix [29–32]. UV rays and aging lead to excess proteolytic activity that disturbs the skin’s three-dimensional integrity . These proteinases are important for breaking down the extracellular matrix during chronic wound repair, in which there is reepithelialization by keratinocyte migration. Thus, MMPs are continuously involved in the remodelling of the skin after chronic damage. Photodamage also results in the accumulation of abnormal elastin in the superficial dermis, and several MMPs have been implicated in this process . ROS activate cytoplasmic signal transduction pathways in resident fibroblasts that are related to growth, differentiation, senescence, and connective tissue degradation . ROS activate cytoplasmic signal transduction pathways that are related to growth differentiation, senescence, transformation and tissue degradation and cause permanent genetic changes in protooncogenes and tumour suppressor genes . The study of Kang et al.  revealed that UVA/UVB irradiation of the skin causes generation of H2O2 within 15 minutes. AP-1, which leads to increased collagen breakdown, becomes elevated and remains elevated within 24 hours following UV irradiation . Decreased procollagen synthesis within eight hours of UV irradiation was demonstrated . Consequently, increased collagen breakdown was demonstrated . It is hypothesized that dermal breakdown is followed by repair that, like all wound repair, is imperfect. Imperfect repair yields a deficit in the structural integrity of the dermis, a solar scar. Dermal degradation followed by imperfect repair is repeated with each intermittent exposure to ultraviolet irradiation, leading to accumulation of solar scarring and ultimately visible photoaging . While it may seem that the signs of photoaging appear overnight, they actually lie invisible beneath the surface of the skin for years (Figure 1). UV exposure of the skin causes oxidative stress, leading to inflammatory reactions, such as acute erythema and chronic damage. Most problematic consequences of chronic damage include premature skin aging and skin cancer .
3. Skin Antioxidants Protect against UVR
UVR exposure affects the skin antioxidants. Ascorbate, glutathione (GSH), superoxide dismutase (SOD), catalase, and ubiquinol are depleted in all layers of the UVB-exposed skin. Studies of cultured skin cells and murine skin in vivo have indicated that UVR-induced damage involves the generation of ROS and depletion of endogenous antioxidants . In the study by Shindo et al. , enzymatic and nonenzymatic antioxidants in the epidermis and dermis and their responses to ultraviolet light of hairless mice were compared. Mice were exposed to solar light and subsequently examined for UV-induced damage of their skin. After irradiation, epidermal and dermal catalase and SOD activities were greatly decreased. Alpha-tocopherol, ubiquinol 9, ubiquinone 9, ascorbic acid, dehydroascorbic acid, and reduced GSH decreased in both epidermis and dermis by 26% to 93%. Oxidized GSH showed a slight nonsignificant increase. Because the reduction in total ascorbate and catalase was much more prominent in epidermis than dermis, the authors concluded that UV light is more damaging to the antioxidant defences in the epidermis than in the dermis. Many other studies confirmed that acute exposure of human skin to UVR in vivo leads to oxidation of cellular biomolecules that could be prevented by prior antioxidant treatment. There have been many studies performed where different antioxidants or combinations of antioxidants and different phytochemicals were tested in order to find evidence against ROS-induced damage. Some of them are presented in Tables 1 and 2.
4. Endogenous Skin Antioxidants
Skin has a network of protective antioxidants. They include endogenous enzymatic antioxidants such as GSH peroxidase (GPx), SOD, and catalase and nonenzymatic low-molecular-weight antioxidants such as vitamin E isoforms, vitamin C, GSH, uric acid, and ubiquinol . All the major antioxidant enzymes are present in the skin, but their roles in protecting cells against oxidative damage generated by UV radiation have not been elucidated. In response to the attack of ROS, the skin has developed a complex antioxidant defence system including, among others, the manganese-superoxide dismutase (MnSOD). MnSOD is the mitochondrial enzyme that disposes of superoxide generated by respiratory chain activity. Of all electrons passing down the mitochondrial respiratory chain, it is estimated that 1% to 2% are diverted to form superoxide (although recent studies claim that this amount is even less); thus, production of hydrogen peroxide occurs at a constant rate due to MnSOD activity. MnSOD dismutates the superoxide anion () derived from the reduction of molecular oxygen to hydrogen peroxide (H2O2), which is detoxified by GSH peroxidase to water and molecular oxygen. The study of Poswig et al.  revealed that adaptive antioxidant response of MnSOD following repetitive UVA irradiation can be induced. The authors provide evidence for the increasing induction of MnSOD upon repetitive UVA irradiation that may contribute to the effective adaptive UVA response of the skin during light hardening in phototherapy. The study of Fuchs and Kern showed that acute UV exposures lead also to changes in GSH reductase and catalase activity in mouse skin but insignificant changes in SOD and GSH peroxidase . The study of Sander et al.  confirmed that chronic and acute photodamage is mediated by depleted antioxidant enzyme expression and increased oxidative protein modifications. Biopsies from patients with histologically confirmed solar elastosis, from non-ultraviolet-exposed sites of age-matched controls, and from young subjects were analysed. The antioxidant enzymes catalase, copper-zinc superoxide dismutase, MnSOD, and protein carbonyls were investigated. Whereas overall expression of antioxidant enzymes was very high in the epidermis, low baseline levels were found in the dermis. In photoaged skin, a significant depletion of antioxidant enzyme expression was observed within the stratum corneum and in the epidermis. Importantly, an accumulation of oxidatively modified proteins was found specifically within the upper dermis of photoaged skin. Upon acute ultraviolet exposure of healthy subjects, depleted catalase expression and increased protein oxidation were detected. Exposures of keratinocytes and fibroblasts to UVB, UVA, and H2O2 led to dose-dependent protein oxidation confirming in vivo results.
Not all skin cells are exposed to the same level of oxidative stress. It was found that keratinocytes utilize as much oxygen as fibroblasts, even though maximal activities of the respiratory chain complexes are two- to five-fold lower, whereas expression of respiratory chain proteins is similar. Superoxide anion levels are much higher in keratinocytes, and keratinocytes display much higher lipid peroxidation level and a lower reduced glutathione/oxidized glutathione ratio .
It can be concluded that oxidative stress is a problem of skin cells and that endogenous as well as exogenous antioxidants could play an important role in decreasing it.
5. Compounds Derived from the Diet with Photoaging/Damage Protective Effects
Natural antioxidants are generally considered to be beneficial fruit and vegetable components. It seems that skin’s antioxidative defence is also influenced by nutritive factors. Besides vitamins A, C, and E, η-3 fatty acids certain nonvitamin plant-derived ingredients might have beneficial effect on skin aging, skin sun protection, or skin cancer. The laboratory studies conducted in animal models suggest that many plant compounds have the ability to protect the skin from the adverse effects of UVR. The proliferation of products, however, can cause confusion among consumers, who often ask their dermatologists for advice as to which antiaging products they should choose. Ideally, the antiaging claims of cosmeceutical formulations and their components should be demonstrated in controlled clinical trials , but there is a lack of such studies due to their high costs. Since cosmeceutical products are claiming that they therapeutically affect the structure and function of the skin, it is rational and necessary to hold them to specified scientific standards that substantiate efficacy claims .
Many studies have found that vitamin C can increase collagen production, protect against damage from UVA and UVB rays, correct pigmentation problems, and improve inflammatory skin conditions  (Table 1).
Topical retinoids remain the mainstay for treating photoaging given their proven efficacy in both clinical and histological outcomes. The application of retinoids might not only clinically and biochemically repair photoaged skin, but their use might also prevent photoaging . Retinoid-mediated improvement of photoaging is associated with increased collagen I synthesis , reorganization of packed collagen fibres , and increased number of type VII anchoring fibrils . However, up to 92% of subjects who used tretinoin in various clinical studies have reported “retinoid dermatitis,” that is, erythema and scaling at the site of application [106, 107]. Irritation can be minimized by reducing dose and frequency of treatments.
It seems that the biochemistry of CoQ10 may inhibit the production of IL-6, which stimulates fibroblasts in dermis by paracrine manner to upregulate MMPs production, and contribute to protecting dermal fibrous components from degradation, leading to rejuvenation of wrinkled skin . It was reported that CoQ10 strongly inhibits oxidative stress in the skin induced by UVB via increasing SOD2 and GPx . It was reported that it is considered that CoQ10 appears to have also a cutaneous healing effect in vivo .
Green tea polyphenols have received attention as protective agents against UV-induced skin damage. Analysis of published studies demonstrates that green tea polyphenols have anti-inflammatory and anticarcinogenic as well as anti-aging properties. These effects appear to correlate with antioxidant properties of green tea polyphenols, which could be used as new photoprotection agents (Table 1).
A number of experimental studies indicate protective effects of beta-carotene against acute and chronic manifestations of skin photodamage. However, most clinical studies have failed to convincingly demonstrate its beneficial effects so far. Studies on skin cells in culture have revealed that beta-carotene acts not only as an antioxidant but also has unexpected prooxidant properties . For this reason, further studies with focus on in vivo β-carotene-induced prooxidative properties and its relevance on human health are needed. Another problem represents the dosage. Although studies convincingly showed that vitamin supplementation effectively protects the skin against sunburn, the doses of vitamins used were generally much higher than amounts generally ingested from habitual diets . Additionally, it was shown that the combination of different antioxidants applied simultaneously can provide a synergistic effect . Antioxidants are most effective when used in combination (Table 2). Vitamin C regenerates vitamin E, and selenium and niacin are required to keep glutathione in its active form. It has been demonstrated that vitamin C can regenerate α-tocopherol from its chromanoxyl radical  and that the vitamin C radical may be recycled by GSH nonenzymatically under slightly acidic conditions  that are present in the stratum corneum . Werninghaus et al.  reported that vitamin E given orally at 400 IU/day for a period of six months affords no significant increase in UV protection. Similarly, in a study with 12 volunteers, vitamin C given at 500 mg/day over eight weeks had no effect on the UV-induced erythemal response , indicating again the importance of antioxidants to be supplemented together to obtain the synergistic effect.
Studies (usually performed on skin cells in vitro or on animal models) suggest that oral uptake of selected micronutrients and phytochemicals can provide photoprotection of human skin . Nevertheless, photoprotection can only be achieved if an optimal pharmacological dose range is reached in the human skin due to well-known prooxidative reactions of antioxidants, for example, in the case of excessive carotenoid concentrations (Table 3). Nevertheless, research is continuously demonstrating that various phytopharmaceuticals offer significant protection against different diseases and skin aging. The primary treatment of photoaging is photoprotection, but secondary treatment could be achieved with the use of antioxidants and some novel compounds such as polyphenols. Exogenous antioxidants like vitamin C, E, and many others cannot be synthesized by the human body and must be taken up by the diet.
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