Research Article
Combination of ERG9 Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene in Saccharomyces cerevisiae
Table 2
Yeast strains and plasmids used in this study.
| | Strain | Genotype | Plasmid | Reference |
| | MTCC 3157 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | None
| Imtech | | YCF-001 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0, erg9::pMET3-ERG9 | None | In this study | | YCF-AD | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 2µ pESC-URA-ADS | pESC-URA-ADS | In this study | | YCF-002 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0, erg9::pMET3-ERG9 pESC-URA-ADS | pESC-URA-ADS | In this study | | YCF-004 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0, FPPS | pY01FPPS | In this study | | YCF-005 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0, erg9::pMET3-ERG9 FPPS-ADS | pY01FPPS-ADS | In this study | | YCF-006 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0, erg9::pMET3-ERG9 ADS-FPPS | pY01ADS-FPPS | In this study |
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