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Journal of Chemistry
Volume 2014 (2014), Article ID 848571, 6 pages
http://dx.doi.org/10.1155/2014/848571
Research Article

Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells

1Life School of Yantai University, Yantai, Shandong 264005, China
2Department of Neurosurgery, Yantaishan Hospital, Yantai, Shandong 264000, China

Received 16 May 2014; Accepted 30 May 2014; Published 18 June 2014

Academic Editor: Wang Chunming

Copyright © 2014 Gang Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study is to evaluate the protection of ginsenoside Rg3 against oxidative stress in human neuroblastoma SK-N-SH cells. 20(R)-ginsenoside Rg3 (20(R)-Rg3) and 20(S)-ginsenoside Rg3 (20(S)-Rg3) were prepared by the method of chemical degradation and column chromatography, and the structure of the two compounds was characterized by 1H-NMR and 13C-NMR spectroscopy. MTT assay and LDH leakage assay were used to determine the cell viability and the oxidative stress cellular model was established by means of H2O2 (600 μM for 4 h). We also investigated the changes of intracellular MDA content, SOD activity, and ROS formation after the treatment of ginsenoside Rg3 for 20 h. The results indicated that both 20 (R)-Rg3 and 20 (S)-Rg3 had obvious protection against H2O2-induced oxidative stress in SK-N-SH cells. Moreover, 20(R)-Rg3 exhibited better antioxidant activity than 20(S)-Rg3 in vitro. These findings are expected to provide some implication for further research and application of ginsenoside Rg3 in neuroprotection.