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Journal of Chemistry
Volume 2015, Article ID 308602, 7 pages
Research Article

Antioxidant Peptide Derived from Spirulina maxima Suppresses HIF1α-Induced Invasive Migration of HT1080 Fibrosarcoma Cells

1Department of Pharmaceutical Engineering, Silla University, Busan 9491, Republic of Korea
2Department of Biomedical Engineering, Pukyong National University, Busan 8160, Republic of Korea
3Department of Chemistry, Pukyong National University, Busan 8160, Republic of Korea

Received 28 July 2015; Revised 7 October 2015; Accepted 8 October 2015

Academic Editor: Eun-Kyung Kim

Copyright © 2015 Won Suk Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Hypoxia causes the malignant progression of tumor cells; hence, it has been considered a central issue that must be addressed for effective cancer therapy. The initiation of tumor metastasis requires invasive cell migration. Here, we show that an antioxidant peptide derived from Spirulina maxima suppresses hypoxia-induced invasive migration of HT1080 human fibrosarcoma cells. HT1080 cells treated with a hypoxia-inducing agent, CoCl2, exhibited an increase in invasive migration and intracellular reactive oxygen species (ROS), which is associated with an increase in the expression of hypoxia-induced factor 1α (HIF1α) accompanied by the activation of PI3K/Akt and ERK1/2. The inhibition of PI3K/Akt and ERK1/2 with specific inhibitors diminished the CoCl2-induced increase in HIF1α expression and invasive cell migration. Moreover, CoCl2-induced HIF1α expression was associated with an increase in the expression of molecules downstream of β-integrin, such as N-cadherin, vimentin, and β-catenin. Therefore, the S. maxima peptide effectively attenuated the CoCl2-induced ROS generation and downregulated the HIF1α signaling pathway involving PI3K/Akt, ERK1/2, and β-integrin in cells. These results suggest that the S. maxima antioxidant peptide downregulates the HIF1α signaling pathway necessary for hypoxia-induced invasive migration of HT1080 cells by attenuating intracellular ROS. S. maxima peptide may be an effective constituent in antitumor progression products.