Research Article | Open Access
Mechanical Stress Results in Immediate Accumulation of Glucosinolates in Fresh-Cut Cabbage
The intensity of mechanical stress and the temperature significantly affect the levels of individual and total glucosinolates in shredded white cabbage (cv. Galaxy). Mild processing (shredding to 2 mm thickness) at 8°C resulted in the accumulation of glucosinolates (40% increase) in comparison with unshredded cabbage, which was already seen 5 min after the mechanical stress. Severe processing (shredding to 0.5 mm thickness) at 20°C, however, resulted in an initial 50% decrease in glucosinolates. The glucosinolates accumulated in all of the cabbage samples 30 min from processing, resulting in higher levels than in unshredded cabbage, except for the severe processing at 20°C where the increase was not sufficient to compensate for the initial loss. Glucobrassicin and neoglucobrassicin were the major glucosinolates identified in the cabbage samples. Mechanical stress resulted in an increase in the relative proportion of glucobrassicin and in a decrease in neoglucobrassicin.
Sales of minimally processed vegetables are rapidly increasing, and these products have become an important convenience food [1, 2]. However, the preparation of fresh-cut products causes damage to the plant tissue, resulting in a more perishable product that has a shortened shelf-life, compared to intact fruit and vegetables . Higher respiration rates and transformation of many nutritionally important components result from the peeling, coring, cutting, shredding, and slicing of fruit and vegetables. These processing operations can lead to biochemical deterioration, surface browning, development of off-flavours, and texture break-down, which often results in lower quality products [4, 5]. Mechanical damage, on the other hand, can induce stress responses that result in the accumulation of desired bioactive compounds .
White cabbage is one of the few vegetables that can be stored until the new harvest, if this is done under the appropriate conditions, and it is an important source of plant bioactive compounds, especially in the winter months. A substantial proportion of cabbage consumption is as the fresh vegetable, as a salad, and its preparation involves intensive mechanical processing (cutting or shredding). Extensive tissue damage results in the decompartmentalisation of the cellular constituents and the mixing of enzymes and substrates. In cabbage and other plants of the Brassicaceae family, a typical, pungent, mustard-like aroma is almost immediately released. Volatile, unstable aglycones are formed after enzymatic hydrolysis of glucosinolates (GLS) by myrosinase, which then rearrange into a range of biologically active, and sometimes toxic, compounds, typically as isothiocyanates and nitriles [7, 8]. These products have potent antimicrobial activities and are toxic to many insect herbivores [9, 10].
Historically, GLS have been regarded as antinutritional factors in human and animal foods . Nevertheless, in more recent years, many nutritional and biochemical studies have indicated beneficial effects of GLS and their degradation products on human health, mostly due to their anticarcinogenic activities [12–14]. Vegetables with high GLS content and an acceptable taste are now desired on the market.
Mechanical stress results in not only the degradation of GLS but also the induction of the enzymes involved in their biosynthesis, which can arise through signalling molecules such as jasmonic acid, salicylic acid, and ethylene . The total GLS content is significantly increased in chopped white cabbage and certain other Brassicaceae when they are left exposed to the air for two days, which is mostly due to the accumulation of indolyl GLS . However, there is no information in the literature relating to the changes in GLS content immediately after processing and the influence of the intensity of the mechanical stress applied.
The aim of the present study was to evaluate the influence of intensity of mechanical damage and of the storage temperature of changes in GLS content in fresh-cut white cabbage. We shredded white cabbage and analysed the changes in GLS content at specific times. The influence of temperature and the severity of the processing were assessed immediately after shredding and after up to one day of storage in air.
2. Material and Methods
White cabbage (Brassica oleracea var. capitata L. forma alba) of cultivar Galaxy was obtained from Janež farm, Sneberje, Ljubljana, Slovenia. The cabbage was harvested approximately 180 days after transplantation with g kg−1 soluble solids content. Samples were transported in the cold-storage chamber, where the cabbage heads were stored at a temperature of 0°C (±1°C) and 97% relative humidity. Twenty-four hours before processing, the cabbage heads were conditioned in thermostated chambers with 97% relative humidity and at 8°C or 20°C. The outer and damaged leaves were removed and the cabbages were processed as follows.
2.2. Experimental Design
The processing of the cabbages started with cutting them into quarters vertically and slicing out the core. The cabbage quarters were then shredded in the thermostated chambers, into slices of different thicknesses, using a kitchen electric slicing machine (Gorenje Food Processor S 201, Slovenia). Aliquots of shredded cabbage were then incubated under the appropriate conditions for predetermined times. All experiments were performed in independent triplicates (cabbage heads). Extraction and analysis of GLS in the individual samples (of independent triplicates) were performed in duplicate. The GLS content was expressed in μmol kg−1 fresh matter. The mass of the shredded cabbage samples was not decreased by more than 2% during storage, indicating only a small moisture loss.
Six cabbages were included in the experiment, three conditioned at 8°C and three at 20°C. Part of each cabbage head was shredded into thick 2 mm slices, and the other part into thin 0.5 mm slices. Aliquots of 200 g of each shredding type were placed into separate jars, which were covered with perforated parafilm to allow the exchange of gases and to minimise water loss by evaporation. Notably here, the temperature of the whole and shredded cabbages was held constant during the whole experiment, at either 8°C or 20°C. Sampling was carried out at the predetermined times of 5 min, 30 min, 2 h, 12 h, and 27 h after shredding. Large patches of a mixture of internal and external leaves were used as the control, which was unshredded cabbage. The samples were then homogenised and subjected to analysis of GLS content by liquid chromatography-mass spectrometry (LC-MS).
2.3. Determination of Glucosinolates Content
The GLS content was determined according to previously published procedures [17, 18], with modifications. Cabbage samples ( g) were transferred into 10 mL methanol (Merck). The suspensions were homogenised (Ultra-Turrax T 25, with dispersing element S25N-18G, Janke & Kunkel, IKA-Labortechnik, Germany) at 10,000 rpm for 60 s in an ice bath. The mixtures were then passed through filter paper (Sartorius 388, FT-3-101-150), and aliquots of the filtrates (2 mL) were centrifuged at 16,000 ×g for 10 min (Eppendorf microcentrifuge 5415D). The supernatants were finally passed through syringe filters (0.45 μm PTFE, Rectek).
HPLC analyses were performed on an Agilent 1100 system, at a temperature of 25°C. The analytical column was a Synergi Hydro-RP (150 mm × 2 mm, particle size, 3 μm) from Phenomenex (Torrance, CA, USA). The separation was performed at a flow rate of 0.250 mL min−1 by gradient elution with 0.1% trifluoroacetic acid (Fluka) in water as solvent A and methanol (Merck) as solvent B. The gradient programme was as follows: 100%–98.3% A, 0–5 min; 98.3%–20% A, 5–15 min; 20% A, 15–17 min; 20%–100% A, 17–20 min; 100% A, 20–30 min. An injection volume of 10 μL was used. GLS were identified and quantified using retention times and the spectra from certified reference materials (BCR-367R, Fluka) of known concentrations, run under the same conditions. The following GLS were quantified: sinigrin, glucobrassicin, neoglucobrassicin, 4-hydroxy-glucobrassicin, glucoalyssin, progoitrin, glucobrassicanapin, gluconapin, gluconasturtiin, and gluconapoleiferin.
The mass-selective detector (Waters, Quattro micro API) was equipped with electrospray ionisation using a cone voltage of 40 V and a capillary voltage of 3.6 kV for negative ionisation of the analytes. The dry nitrogen was heated to 350°C and the drying gas flow was 400 L h−1. The cone gas flow (nitrogen) was 50 L h−1. The data were acquired in the selected ion mode (sinigrin, 358.20; glucobrassicin, 447.13; neoglucobrassicin, 477.19; 4-hydroxy-glucobrassicin, 463.19; glucoalyssin, 450.19; progoitrin, 388.10; glucobrassicanapin, 386.41; gluconapin, 372.09; gluconasturtiin, 422.19; gluconapoleiferin, 402.41). The reproducibility of the GLS content was established by analysing the same (random) sample in six replicates; the coefficient of variation for all was lower than 6.4%.
2.4. Statistical Analysis
The experimental data were evaluated statistically using the SAS/STAT programme. Basic statistical parameters were calculated by the MEANS procedure. The data were tested for a normal distribution and analysed by the general linear model. The statistical model included the main effects of shredding type (thin or thick) and storage time (unshredded, 5 min, 30 min, 2 h, 12 h, and 27 h after shredding), interaction of type and time, and repetition (1–3). Means for the experimental groups were obtained using the Duncan procedure and were compared at the 5% probability level.
3. Results and Discussion
3.1. Content of Glucosinolates in Unshredded White Cabbage
GLS in all of the samples were analysed by LC-MS (Figure 1). The average content of total GLS in the unshredded cabbage was μmol kg−1 fresh weight (FW). Two indolyl GLS, glucobrassicin ( μmol kg−1 FW) and neoglucobrassicin ( μmol kg−1 FW), accounted for more than 75% of all of the GLS identified. Among the other GLS, only the content of the aliphatic GLS sinigrin ( μmol kg−1 FW) was high ≈10%. Minor quantities of gluconasturtiin ( μmol kg−1 FW), glucobrassicanapin ( μmol kg−1 FW), 4-hydroxy-glucobrassicin ( μmol kg−1 FW), progoitrin ( μmol kg−1 FW), glucoalyssin ( μmol kg−1 FW), and gluconapoleiferin and gluconapin (under 1 μmol kg−1 FW) were also identified. A survey of the available data in the literature revealed that the content of total GLS in white cabbage can be within the span of one order of magnitude, as various studies have reported values in the range of 300 μmol kg−1 FW to 3000 μmol kg−1 FW [16, 19–23]. Large variations in the content of total GLS and in their composition have been observed between different cultivars, geographical regions, times and conditions of storage, and sampling seasons and climates [21, 24, 25]. The data obtained in the present study are well within this range. A relatively large biological variability was reflected in the high coefficient of variation (30%), as also observed in other studies. Appropriate control experiments are therefore extremely important when the influence of processing on GLS content is assessed.
3.2. Influence of Intensity of Shredding and Storage Temperature on Glucosinolate Content in White Cabbage, to 27 h
The shredding intensity and temperature already had large effects on total GLS content within 5 min of processing. Mild processing at 8°C (Table 1) resulted in an immediate and statistically significant increase in GLS content, as ≈40% higher levels of GLS were revealed in the processed cabbage, in comparison with the unshredded cabbage. When thin shredded cabbage that was processed at 8°C was analysed after 5 min, no changes were seen for the total GLS. Processing of the cabbage at 20°C resulted in a lower total GLS content immediately after shredding (Table 2), in comparison with the same intensity of mechanical stress at 8°C. A statistically significant decrease (50%) was observed for the thin shredded cabbage 5 min after processing, whereas mild processing did not result in the higher total GLS content that was observed at the lower temperature.
|Content expressed as means ± standard deviation. Means with different letters within a row (, , ) differ significantly (; significance of difference between measuring times). Means with different letters within a column (, ) differ significantly (; significance of difference between shredding types). Thin, 0.5 mm slices; thick, 2 mm slices.|
|Content expressed as means ± standard deviation. Means with different letters within a row (, , , ) differ significantly (; significance of difference between measuring times). Means with different letters within a column (, ) differ significantly (; significance of difference between shredding types). Thin, 0.5 mm slices; thick, 2 mm slices.|
Analysis of total GLS content 30 min after processing revealed that higher GLS contents were determined under all conditions, in comparison to the GLS content in cabbage determined after 5 min. The increase in total GLS in the time range from 5 min to 30 min was the most pronounced and statistically significant for the thin shredded cabbage processed at 20°C (57%, Table 2). Under the other three conditions (thick at 20°C, thin at 8°C, and thick at 8°C all at 30 min), the total GLS accumulated in comparison with unprocessed cabbage was 22% more GLS for thick shredding at 20°C (Table 2), 25% for thin shredding at 8°C, and 43% for thick shredding at 8°C (Table 1). The changes in the total GLS content were less pronounced during further storage up to 27 h. At the end of the incubation, a statistically significant decrease was only seen for the thin shredded cabbage at 20°C.
The three major glucosinolates, glucobrassicin, neoglucobrassicin, and sinigrin, accounted for more than 85% of the total GLS under all of the conditions analysed. Their contributions to the pool of total GLS changed as a result of the mechanical stress. Under all four processing and storage conditions, the relative proportion of neoglucobrassicin had already decreased 5 min after shredding. On the contrary, the relative proportion of glucobrassicin increased. Further storage at 8°C did not have a significant influence, whereas at 20°C a decrease in the relative proportion of glucobrassicin was observed, accompanied by an increase in neoglucobrassicin, for the thin and thick shredded cabbage after 27 h (Table 3).
|Levels of significance: statistically significant: 5 and ; highly statistically significant: .|
Analysis of the data presented in Tables 1 and 2 reveals the great complexity in the GLS transformation that was induced by the mechanical stress. The extensive tissue damage caused by the thin shredding was reflected in an immediate decrease in GLS content at 20°C. Disruption of GLS-containing S-cells and myrosinase-containing M-cells will result in colocalisation of this enzyme and its substrates, which leads to substrate hydrolysis . The decrease in GLS content that was observed only at 20°C can be attributed to a higher reaction rate, which is typically more than doubled when the temperature is increased by 10°C within this temperature range.
There have been various studies on the influence of mechanical stress on GLS content during prolonged storage of Brassicaceae [15, 16, 27, 28]. Those studies were performed on relatively large time scales, with the first measurements typically determined only the day after the processing. However, these data have been controversial, as both increases and decreases in GLS have been reported. The influences of biotic and abiotic stress on the induction of enzymes and other proteins involved in GLS biosynthesis at the DNA level are well documented , and these can explain the accumulation of GLS that has been observed in some studies. In the present study, we analysed the changes in GLS content immediately after processing and the increase observed within a 30 min period, under all four processing and storage conditions. However, at these shorter times, the mechanisms of enzyme induction cannot explain the effects, as the time is too short, and thus other mechanisms must be involved.
The key step in GLS biosynthesis is the formation of aldoximes from their precursor amino acids . Mechanical stress results in accumulation of reactive oxygen species and an increase in H2O2 levels . The enzyme that catalyses the conversion of tryptophan into indole-3-acetaldoxime, the precursor of indole glucosides, is peroxidase, which is stimulated by H2O2 . The pool of precursor free amino acids in Brassicaceae such as broccoli and white cabbage is relatively small, although they are sufficient to compensate for the loss of GLS by hydrolysis [33, 34]. However, the content of neoglucobrassicin formed by a secondary modification of glucobrassicin is not increased in line with this hypothesis. Thick shredding resulted in higher GLS content at both temperatures. Under these conditions, fewer cells were disrupted, and accordingly biosynthesis prevailed over GLS hydrolysis.
It appears obvious that the shredding intensity has a large influence on GLS content immediately after the processing of white cabbage. Intensive tissue damage at 20°C results in a lower GLS content, whereas mild processing at 8°C results in the accumulation of GLS even only 5 min after shredding. Further storage for up to 30 min resulted in the accumulation of GLS in comparison to the GLS content after 5 min, for all of these processing conditions. The observed increase in GLS content was within a timescale that is too short to be explained by enzyme biosynthesis at the DNA level. The relative proportions of the two major glucosinolates, neoglucobrassicin and glucobrassicin, were also changed after shredding, in favour of higher glucobrassicin levels.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
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