Purification and Characterization of a New Lectin from Loach Skin Mucus

Figure 1

(a) Purification of lectin from loach skin mucus by DEAE-52: the four fractions (D-0, D-1, D-2, and D-3) were eluted by 0 (PBS, 20 mM, pH 7.2), 0.3, 0.5, and 0.7 M·NaCl solution (mixed with PBS, 20 mM, pH 7.2) at a flow rate of 1 mL/min, respectively; (b) purification of D-3 by Sephadex G200: the three fractions were eluted using PBS (20 mM, pH 7.2) solution at a flow rate of 0.3 mL/min.

(a)

(b)